| Project/Area Number |
60570547
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Hematology
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| Research Institution | Hokkaido University |
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Principal Investigator |
KONDO Takahito Hokkaido University School of Medicine, Instructor, 医学部, 助手 (00158908)
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| Project Period (FY) |
1985 – 1986
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| Project Status |
Completed (Fiscal Year 1986)
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| Budget Amount *help |
¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1986: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1985: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | Erythrocyte / Carbonic anhydrase I / Primary aldosteronism / グルタチオン |
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| Research Abstract |
A low activity form of carbonic anhydrase I was found in erythrocytes of five patients with primary aldosteronism. The specific activity was very low, however the activity was restored by drug treatment as well as adrenalectomy.
The enzyme was purified to homogeneity from one of the patients. The enzyme was not distinguishable with respect to its antigenecity and zinc content. On theother hand, the inhibition constant and binding affinity to a specific inhibito, acetazolamide, was quite different. Sulfhydryl titration, amino acid analysis and peptide mapping analysis suggested that the low activity from contains a mixed disulfide with glutathione which is closely associated with its low activity form. This result supports our previous findings on <gamma> -glutamyl cyclotransferase and N-acetyl hexosaminidase, both of which were found to contain a mixed disulfide with glutathione. The presence of a mixed disulfide in the protein seems to be a common phenomenon.
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