Studies on biologically active factors derived from myeloma cells
Project/Area Number |
60570557
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Research Category |
Grant-in-Aid for General Scientific Research (C)
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Allocation Type | Single-year Grants |
Research Field |
Hematology
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Research Institution | Kyoto University |
Principal Investigator |
KANOH Tadashi Faculty of Medicine, Kyoto University, 医学部, 講師 (50093224)
|
Project Period (FY) |
1985 – 1986
|
Project Status |
Completed (Fiscal Year 1986)
|
Budget Amount *help |
¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1986: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1985: ¥1,400,000 (Direct Cost: ¥1,400,000)
|
Keywords | Myeloma Cells / Myeloma / Immunoglobulin / Biologically Active Factors / Antibody-producing system / Phagocytic Myeloma Cells / アミロイド線維 |
Research Abstract |
In this study a variety of biologically active factors derived from myeloma cells have been examined. 1. A factor with a molecular weight of about 300,000 from myeloma cell lines inhibited the differentiation of normal B lymphocytes directly, but not through macrophage. 2. Phagocytosis by myeloma cells was associated with activation of acid phosphatase. On the other hand, myeloma cells occasionally showed prominent vacuoles in the cytoplasm. The acid phosphatase activity was confirmed around and within vacuoles. These vacuoles were considered to be phagic. Thus, phagocytizing ability and vacuolar formation in myeloma cells both of which were associated with an increased acid phosphatase activity express another function of myeloma cells. These functions seem to be closely connected with malignant transformation of plasma cells. 3. An intracellular formation of amyloid fibrils was demonstrated in myeloma cells on the basis of cytochemical, immunochemical and electron microscopic studies. Such phenomenon also could be related to phagocytizing ability of myeloma cells. 4. After this, an effect of myeloma cell-derived factors on other blood cells should be examined.
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Report
(1 results)
Research Products
(10 results)