| Budget Amount *help |
¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1986: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1985: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Research Abstract |
We attempted to study chromosomes with high resolution banding and to find out correlations between chemosomes and clinical features in hematological malignancies.
To obtain elongated and finely banded chromosomes ethidium bromide was added to the cultures of human bone marrow cells for the last 2 hours at a final concentration of 10 ug/ml.
Materials consisted of 32 patients with acute nonlymphocytic leukemia (ANLL). Clonal abnormalities were found in 27 cases (84%) of these cases. The ratio of chromosome abnormalities did not differ among 6 FAB subtypes of ANLL. Specific structual abnormalities were closely related to certain types of leukemia, i.e., t(8;21) for M2, t(15;17) for M3, inv(16) for M4Eo, and t(9;11) for M5.
Other 32 cases of myelodysplatic syndrome (MDS) were examined as well. Chromosome abnormalities were seen in 19cases (59%). Every subtype of MDS showed almost the same abnormality rate. Chromosome aberrations of 5q- were seen in 5 cases, -7/7q- 5 cases, +1q 3 cases, 17p+ 3 cases, and two or more of these abnormalities were frequently seen in the same patients. Any of these alterations did not seem to be specific for certain subtypes of MDS but probably be common findings for MDS.
These results suggest that in ANLL most, if not all, patients have a chromosomal defect when a satisfactory number of metaphases are examined with high resolution banding analysis. However, we could not find out any chromsome abnormalities in approximately 40 % of patients with MDS despite a considerable number of mitoses were analysed. This may suggest that a disease entity of MDS is heterogeneous and some non-neoplastic hematological diseases are included.
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