| Budget Amount *help |
¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1986: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1985: ¥1,300,000 (Direct Cost: ¥1,300,000)
|
|---|
| Research Abstract |
1. Methods for the detection of antineutrophil antibodies (ANAA). Of several methods for the ANA detection studied, we established a most sensitive and objective immunofluorescence (IF) test using a flow cytometer (FACS 420), which can measure neutrophil-reactive IgG (NRIgG) semi-quantitatively. Patients who showed positivity for the direct and auto indirect IF tests were diagnosed as having autoimmune neutropenia (AIN). However, since positivity only for the direct IF test was observed in some patients with other diseases than AIN including aplastic anemia, its specificity remains to be further investigated. 2. Clinical aspects of AIN. Characteristic features of clinical and laboratory findings in 20 patients with AIN were as follows; seldome occurrence of severe infections, normal or increased neutrophil precursor cells with a marked decrease in segmented neutrophils in the bone marrow, a marked decrease in the marrow neutrophil reserve andser increased serum lysozyme levels, suggest
… More
ing increased destruction of neutrophils. The features of 8 patients with chronic idiopathic neutropenia (CIN) which were defined as chronic neutropenia with negative results for both the direct and autoindirect IF tests and the agglutination test were compared to those of AIN: the degree of decreases in the marrow neutrophil reserve and the serum lysozyme levels in CIN were significantly lower than in AIN in spite of no difference in the blood neutrophil counts, suggesting that destruction of neutrophils, if increased, is less pronounced in CIN, and that CIN is a heterogenous disorder. Corticosteroid therapy produced good responses in all the 10 patients with AIN treated, and high-dose intravenous immunoglobulin therapy produced a rapid and transient response in 5 of 7 patients. The former may be indicated for the patients with recurrent infections, and the latter for the patients at severe infections. 3. Natures and characteristics of ANA. NRIgG in serum in all the patients with AIN were polyclonal. The determination of subclasses of the serum NRIgG in AIN showed that IgG3 and IgG1 were positive in a high proportion of the patients, which may correspond to the findings that complement-dependent cytotoxicity test was positive in most of the patients. Neutrophil-antigen (N system) specificity of serum NRIgG could not be identified in 7 patients with AIN tested, while NA2 or NC2 specificity was identified in 2 patients with alloimmune neonate neutropenia. Less
|
