Evaluation of two color flow cytometric analysis of peripheral immunocompetent cell phenotypes as an immunomonitoring for kidney allografted patients
| Project/Area Number |
60570577
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
General surgery
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| Research Institution | University of Tsukuba |
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Principal Investigator |
FUKAO Katashi University of Tsukuba, Institute of Clinical Medicine, Associate Prof., 臨床医学系, 助教授 (50091921)
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| Co-Investigator(Kenkyū-buntansha) |
NOGUCHI Atsuo University of Tsukuba, Institute of Basic Medical Sciences. Assistant Prof., 基礎医学系・免疫学, 講師 (80091916)
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| Project Period (FY) |
1985 – 1986
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| Project Status |
Completed (Fiscal Year 1986)
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| Budget Amount *help |
¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1986: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1985: ¥900,000 (Direct Cost: ¥900,000)
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| Keywords | monoclonal antibody / flowcytometry / peripheral immunocompetent cell / kidney allo-transplantation / 二重染色法 |
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| Research Abstract |
Reliable immunological monitoring method useful for the management of kidney transplanted patients has been required. Flowcytometric analysis of peripheral immunocompetent cell phenotypes using monoclonal antibody had been expected to be an ideal monitoring method.
In this study, we evaluated the single color and two color flowcytometric analysis of peripheral mononuclear cell phenotypes in the management of tranplant patients. Peripheral nucleated cells of 41 kidney transplanted patients, 34 dialyzed chronic renal failure patients, 41 healty adults were examined. Cell phenotypes were analyzed using Leu series monoclonal antibodies conjugated FITC of PE and flowcytomter(FACS IV). In two color assay, we quantitated activated pan T (Leu4+DR+), activated pan T (Leu4+I12+), activated T suppressor/killer(Leu2a+DR+), T suppressor(Leu2a+15+), T killer(Leu2a+1-), T suppressor inducer(Leu3a+8+), T helper(Leu3a+8-), activated monocyte(LeuDR+M3+).
Activated T suppressor/killer ratio in total cells
… More
of transplanted patients were higher than normal control 12 month after transplantation. T suppressor ratio of transplanted patients without acute rejection were higher than those with it, especially in the period between 3 to 12 months after surgery.
T suppressor inducer 12 months after transplantation was lower and activated monocytes of transplanted patients was higher than normal control and chronic renal failure patients. Kinds of immunosuppressant did not paticular affect on the subsets ratio. Time series analysis could not find any common correlation between the alteration of cell subsets and laboratory data or clinical course. At present, we concluded that the quantitation of peripheral immunocompetent cell subsets were not so useful in management of transplanted patients. We developed new assay of phagocytic activity of macrophage using monoclonal antibody, fluorescence latex beads and flowcytometry. Now we are studying this new assay for useful monitoring for management of transplanted patients. Less
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Report
(1 results)
Research Products
(8 results)
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[Publications] 岩崎秀生,深尾立,湯沢賢治,大塚雅昭,岩崎洋治,加納勝利,小磯謙吉,中沢正樹,尾崎梓,野口淳夫,柏原英彦,高橋公夫,太田和夫: 移植. 21. 205-212 (1986)
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