| Project/Area Number |
60570582
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
General surgery
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| Research Institution | The University of Tokyo |
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Principal Investigator |
AKIYAMA Nobuo The Institute of Medical Science, 医科学研究所, 教授 (80012748)
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| Co-Investigator(Kenkyū-buntansha) |
MITA Kunji The Institute of Medical Science, 医科学研究所, 助手 (30190672)
DEGAWA Hisakazu The Institute of Medical Science, 医科学研究所, 助手 (10172115)
TAKEDA Yasushi The Institute of Medical Science, 医科学研究所, 助手 (30163421)
TOMIKAWA Shinji The Institute of Medical Science, 医科学研究所, 助手 (40164016)
YAMAUCHI Jun The Institute of Medical Science, 医科学研究所, 助手 (30012769)
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| Project Period (FY) |
1985 – 1986
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| Project Status |
Completed (Fiscal Year 1986)
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| Budget Amount *help |
¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1986: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1985: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Keywords | Renal transplantation / class <II> antigens / ADCC / K cell activity / ABC method / Monoclonal antibody |
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| Research Abstract |
Our previous study with EB virus-induced lymphoblastoid cell lines ( LCL ) as target cells indicated that human class <II> antigens ( HLA-D antigen ) might be one of the target antigens in cell-mediated lympholysis ( CML ). Antibody-dependent cell-mediated cytotoxicity ( ADCC ) has been undrestood as cytotoxicity shown by nonimmune lymphoid cells at high dilution of IgG antibodies. In the present study, we examined ADCC against human class <II> antigens by using LCL as target cells. The results of our study revealed that HLA-DRantigen was one of the real target antigens in human ADCC. In azathioprine-treated renal transplant recipients, both K cell and NK cell activities were depressed to the level of one half of the normal control subjects, and K cell acitvity in ciclosporin-treated patients was suppressed completely, While NK cell activity was not affected.
Sera collected from renal transplant recipients before and after transplantation were examined. ADCC activity in the pretransplant sera seemed to predict the appearance of anti-donor T and B antibodies detected by complement-dependent cytotoxicity ( CDC ) and the occurrence of severe acute rejection episodes after transplantation.
The possible role of ADCC against class <II> antigens in renal allograft rejection has been suggested by the following facts: A substantial number of mature B lymphocytes secreting IgG antibodies could be recognized with ABC method in the allograft biopsy specimen: HLA-DR antigen was strongly induced on renal tubular cells after rejection and this antigen seemed to be the appropriate targets in ADCC
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