Study on the mechanism of immobilized enzyme activity and clinical application
| Project/Area Number |
60570589
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
General surgery
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| Research Institution | Osaka University Medical School |
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Principal Investigator |
MORI Takesada (1987) Professor Osaka Univesity Medical School., 医学部, 教授 (60028496)
大城 孟 大阪大学, 医学部, 講師 (30028542)
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| Co-Investigator(Kenkyū-buntansha) |
OHSHIRO Takeshi. Assistant Professor Osaka University Medical School., 医学部, 講師 (30028542)
KAMBAYASHI Junichi. Research Fellow Osaka Univesity Medical School., 医学部, 助手 (40135687)
森 武貞 大阪大学, 医学部, 教授 (60028496)
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| Project Period (FY) |
1985 – 1987
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| Project Status |
Completed (Fiscal Year 1987)
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| Budget Amount *help |
¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1987: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1986: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1985: ¥700,000 (Direct Cost: ¥700,000)
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| Keywords | Urokinase / Tissue plasminogen activator / Immobilized enzyme / prosthetic graft / MCA法 / 腎組織由来ウロキナーゼ / 組織プラスミノゲンアクチベータ |
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| Research Abstract |
One of the methods of preparing an antithrombogenic material is to immobilize a fibrinolytic enzyme on the surface of a carrier.
In this project, we studied the mechanisms of immobilized enzyme activity and clinical applications.
The materials used in this experiment were tissue-culture-urokinase and tissue-plasminogen activator as enzyme, and nylon and polyurethane as carrier. Immobilization was performed by peptide linking procedure.
These results were as follow
1. Immobilized enzymes maintained the high fibrinolytic activity after 6 months storage.
2. The activities were relatively stable even against the inhibitor and antibody.
3. Enzyme immobilized materials had the effective antithrombogenic ability in vitro and in vivo.
4. But, the fibrinolytic activity decreased gradually after a prolonged catheterization period.
5. Now, these antithrombogenic catheters have been utilized in clinical cases with IVH and TPN for the Investigations.
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Report
(2 results)
Research Products
(12 results)
