| Project/Area Number |
60570614
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Digestive surgery
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| Research Institution | Asahikawa Medical College |
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Principal Investigator |
HAYASHI Hirokazu Asahikawa Medical College, Assistant, 医学部, 助手 (90133834)
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| Co-Investigator(Kenkyū-buntansha) |
EBATA Hidetaka Asahikawa Medical College, Associate Professor, 医学部, 助教授 (20091564)
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| Project Period (FY) |
1985 – 1986
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| Project Status |
Completed (Fiscal Year 1986)
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| Budget Amount *help |
¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1986: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1985: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | pancreas islet isolation technique / Freeze Disruptive Separation Method / hybridoma / <VX_2> -A cell line / Ultra-rapid colling method / 超急速細胞凍結法 / 人工膵臓 |
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| Research Abstract |
Four Progects were done by this grant:
First of all, A new isolation method of the islets from the pancreas without collagenase ddigestion was developed and named "Freeze Dissuptive Separation Method".
Second, A new cultured cell line, <VX_2> -A Asahikawa/, in which a planned partner of an islet cell hybridoma was established. But, insulin-releasable hybridoma was not established.
Third, An extra-corporal artificial pancreas was examined.
Finally, A new cryopreservation system for isolated pancreas islets and cultured cells was developed. This system is an ultra-rapid cooling method using liquid helium that is available in any laboratory and of minimal cost.
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