| Budget Amount *help |
¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1986: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1985: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Research Abstract |
Fatty acid binding protein (FABP) is present in the cytosol of mammalian intestine, liver and other tissues, which has a high affinity for long chain fatty acids as well as for Bilirubin and other dyes. It has been suggested that FABP is related to fatty acid transport and metabolism in those tissues.
We found that anti-body to liver (L-) FABP which isolated and purified from rat liver cytosol, cross-reacts immunologically against intestinal mucosal homogenate of man. From this point of view, we studied the distribution of L-FABP in human gastrointestinal tract by Ouchterlony double diffusion and Peroxidase-anti-peroxidase methods. And then we Quantified the content of L-FABP in the parts where it existed by single radial immunodiffusion method.
Results were as follows: L-FABP was present in duodenum, jejunum, ileum, and colon, exept in esophagus and stomach. It mainly existed in epithelial absorptive cells of mucosa, significantly greater in villi than in crypts. Supernatant L-FABP concentration in mucosa of jejunum, ileum, and colon were similar, expressed as micrograms per milligram soluble protein, and smaller amount of L-FABP was present in duodenum. L-FABP content varied according to dietary conditions and diseases.
These findings are considered to support the concept that L-FABP participates in fatty acid transport and utilization. It is interesting with regard to its function that large amount of L-FABP was present in ileum and colon of man as much as in jejunum, in relation to intestinal malabsorption of fat.
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