Experimental Study on Nerafts
| Project/Area Number |
60570711
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Osaka Medical College |
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Principal Investigator |
ABE Muneaki Osaka Medical College,Department of Orthopedic Surgery, 整形外科, 助教授 (70084994)
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| Co-Investigator(Kenkyū-buntansha) |
KINOSHITA Mituo Osaka Medical College,Department of Orthopdic Surgery, 整形外科, 講師 (90169912)
DOHI Tsuneo Osaka Medical College,Department of Orthopedic Surgery, 整形外科, 助手 (70188853)
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| Project Period (FY) |
1985 – 1987
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| Project Status |
Completed (Fiscal Year 1987)
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| Budget Amount *help |
¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1987: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1986: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1985: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Keywords | vascularized nerve qraft / denervated nerve qraft / immunohistochemistry neurofilament / myelin basic protein / 塩基性蛋白 / S-100蛋白 |
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| Research Abstract |
The regenerative and degenerative changes of the axons,Schwann ceiis and myelin sheaths were observed using an immunohitochmical techique three mode: 1,vascularized 2,free denervated 3 free fresh nerve qraft. 1.One week after the operation,granular debris with NF immunoreactivety was observed in the clear space in the qrouqs V and F. In group D, however,decreased of MBP immunoreactivity and no positive stain for S-100 immunoreactive changes 2.After 2 weeks,regenerating axons with NF immunoreactvity were observed in qroups V and F. 3 weeks, reqeneratyve axons were opserved in all cases in qroups D-1 and D-3, but only in 2 of 5 cases in qroup D-6. Little,MBP immunoreactivity in the clear space was observed in each qroup. 4. Six weeks after the operation regenerating axons with NF immunoreactivity had projeccted past the transplantation reqion in all the animals in qroups F, V and D, Axons with NF immunoreactivity were observed in central and peripheral portions in qroup V, but in qroup D, they were seen only in the peripheral portion. 5. After 12 weekds, motor conduction velocity (mean<plus-minus>SD) was 18.8<plus-minus>1.5m/sec in qroup F and 27.6<plus-minus>4.0m/sec in qroup V,no was detected in group D.mbp and S-100 immunoreactivities were qreater in qroups F and V than in qroup D. 6. According to our sxperiment,the vascularized nerve qraft appers top be useful in the treatment of peripheral nerve injSi-ta-
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Report
(2 results)
Research Products
(5 results)
