| Project/Area Number |
60570723
|
|---|
| Research Category |
Grant-in-Aid for General Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
麻酔学
|
|---|
| Research Institution | KUMAMOTO UNIVERSITY |
|---|
Principal Investigator |
SUGAHARA Kazuhiro Kumamoto University Medical School, 医学部, 講師 (20171126)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
馬場 知子 熊本大学, 医学部, 助手 (60181038)
USHIJIMA Kazuo Kumamoto University Medical School, 医学部, 助手 (60136752)
TSUNO Kyoji Kumamoto University Hospital, 医学部附属病院, 講師 (40163860)
BABA Tomoko Kumamoto University Medical School
|
|---|
| Project Period (FY) |
1985 – 1986
|
| Project Status |
Completed (Fiscal Year 1986)
|
| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1986: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1985: ¥1,800,000 (Direct Cost: ¥1,800,000)
|
|---|
| Keywords | Alveolar type <II> epithelial cells / Surface active material / Lung injury / Repair / Growth factor / 人体材料 |
|---|
| Research Abstract |
1. Cell isolation and culture: Alveolar type <II> cells were isolated from adult rat lungs by tissue dissociation with elastase and density gradient. The yield of the cells is about 20x <10^6> cells/rat and the purity of the adherent cells is 85-95 % type <II> cells. The cells appear similar lamellar bodies and organella to those in intact lung, by tannic acid stain and electron microscope. At the cytoskeleton structure examining by avidin-biotin stain, they have fine intermediate filaments such as keratin and actin. Those structure was affected by different substrates, especially fibronectin.
2. Function of the cells: Alveolar type <II> cells form domes after confluent, which suggests that they have active ion transport. Alveolar type <II> monolayers on filter have bioelectronic properties of potential different(PD);-1.0 mV, resistance(R); 200 ohm・ <cm^2> ; short circuit current(SCC); 1.8 uA/ <cm^2> . The monolayers on fibronectin-coating filter have higher PD and R than those on the f
… More
ilter. Taken together, fibronectin is an important functional component of the extracellular matrix that supports alveolar type <II> cells during alveolar reepithelization.
3. Surface active material secretion: To clarify the mechanism of surfactant secretion, we examined the effects of <beta> -stimulant and <Ca^(++)> on surfactant secretion.
4. An animal model of lung injury: Exposure to 85 % oxygen rats had the proliferation and hypertrophy of alveolar type <II> cells. We have tried to find a factor which induces cell proliferation.
5. Isolation of human alveolar type <II> cells from resected human lung.
6. In vivo culture: Although the cells are necessarily damaged and lose surface receptors to some extent by the isolation procedure and conditions in vitro, we are trying to develop a new system of in vivo culture, which isolated cells are again implanted into the peritoneum or subcutaneous region of the different rat.
7. Function of tracheal epithelium: The tracheal epithelial cells have some similar functions to those of alveolar type <II> epithelial cells, such as ion transport , mucus secretion and proliferation. Less
|
