| Project/Area Number |
60570764
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Asahikawa Medical College |
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Principal Investigator |
ASAKAWA Takehito Asahikawa Medical College, OB & GYN, 医学部, 助手 (20133811)
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| Project Period (FY) |
1985 – 1986
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| Project Status |
Completed (Fiscal Year 1986)
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| Budget Amount *help |
¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1986: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1985: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Keywords | Chromosome / Polyspermy / In vitro fertilization |
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| Research Abstract |
1. In chromosomal studies, 94 (77.7%) of 121 oocytes fertilized in vitro had a normal diploid complement of chromosomes (2n=44) and 6 (5%) showed aneuploidy. The incidense of triploid (dispermy) after in vitro fertilization was higher than the rate in vivo (16.3% V.S. 9.0%).
2. The analysis of in vitro fertilized rabbit embryos reavealed that the incidence of polyploydy (polyspermy) was not effected on by PMS 100 i.u. given to the female.
3. The mucin layer surrounding the rabbit oocytes was not effective to block the polyspermic fertilization in vitro.
4. A 100 fold range of capacitated sperm concentrations ( <10^4> , <10^5> , <10^6> /ml) has been tested to determine whether there is a relationship between the capacitated sperm concentration and the the incidence of polyspermic fertilization in vitro of rabbit oocytes. The sperm concentration in the range of <10^4> - <10^6> /ml gave fertilization rate of around 70%. The frequency of polyploidy (polyspermy) was dependent on the sperm concentration. The rate of polyspermy with <10^4> /ml spermatozoa was the lowerest (9.3%), compared to 14.1% with <10^5> /ml and 13.2% with <10^6> /ml.
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