| Project/Area Number |
60570795
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Keio University |
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Principal Investigator |
KOBAYASHI Toshifumi Keio University, School of Medicine, Dept. of OB/GYN, Associate Professor, 医学部, 助教授 (30051460)
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| Project Period (FY) |
1985 – 1986
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| Project Status |
Completed (Fiscal Year 1986)
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| Budget Amount *help |
¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1986: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1985: ¥900,000 (Direct Cost: ¥900,000)
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| Keywords | IVF-ET / fertilization / oocyte maturation / follicular fluid / perfused ovary / transient hyperprolactinemia / steroidgenesis / 卵巣灌流法 |
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| Research Abstract |
In human in vitro fertilization - embryo transfer(IVF-ET ), most of ova from small follicles under 1 ml of follicular fluid were suggested to be immature. Fertilization and cleavage of ova were relatively good from follicles of 3 or more of follicular fluid.
Transient hyperprolactinemia was generally noted in women treated with hMG. In this study prolactin levels were well-correlated with serum estrogen levels. There were no differences in fertilization and cleavage rates of ova between women with higher serum prolactin levels and those with lower prolactin levels. There was even higher pregnancy rates in women with higher prolactin levels. It was, therefore, suggested that transient hyperprolactinemia seen in women at IVF-ET programs had no effect on oocyte nor follicle maturation.
Cyclic AMP has inhibitory effect on oocyte maturation in mammalia. In this study, cAMP also inhibited maturation of cultured rabbit cumulusenclosed oocytes. Short term exposure of cAMP on oocyte in rabbit ovaryperfusion system even promoted the resumption of oocyte maturation.
Addition of aminoglutethimide to perfusion system had no effect on germinal vesicle breakdown of rabbit ova, but significantly inhibited fertilization rates. Thus it was suggested that steroidogenesis in granulosa cells especially in cumulus cells might play an important role in cytoplasmic maturation of oocyte.
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