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"Genesis and development of meddle ear cholesteatoma, epithelial-mesenchymal interaction in Vitoro"

Research Project

Project/Area Number 60570822
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Otorhinolaryngology
Research InstitutionThe Jikei University School of Medicine

Principal Investigator

SANO Shinichi  The Jikei University School of Medicine,, 医学部, 講師 (80057059)

Co-Investigator(Kenkyū-buntansha) KAMIDE Yousuke  The Jikei University School of Medicine,, 医学部, 助手 (10177579)
Project Period (FY) 1985 – 1986
Project Status Completed (Fiscal Year 1986)
Budget Amount *help
¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1986: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1985: ¥900,000 (Direct Cost: ¥900,000)
Keywordsmiddle ear cholesteatoma / cell proliferation / cell culture / 細胞相互作用
Research Abstract

The development of the meddle ear cholesteatoma arises from the mitotic activity of epithelial cells induced by inflammation. We have established epitherial cell lines from the cholesteatoma(CH-Ep), fibro like cell lines from the choelsteatoma(CH-Fi), epithelial cell lines from the human fetal tympanic membrane(F-Ep) and fibro-like cell lines from human fetal tympanic membrane(F-Fi) in Vitro. We are able to detect synthesis of DNA and protein in each cell by using either <^3H> -tymidine or <^3H> -proline isotoes. The synthesis of DNA and protein in either CH-Ep or F-Ep does not change in the presence of LPS( <10^(-4)> <10^(-10)> g/ml). However the synthesis of DNA and protein in CH-Fi increases in the presence of LPS( <10^(-6)> 5x <10^(-11)> g/ml). The conditioned medium of CH-Fi stimulated by LPS(50ug/ml), increases the synthesis of DNA and protein in CH-Ep. Whereas, the conditioned medium of F-Fi, stimulated by LPS(50ug/ml) does not increase the synthesis of DNA and protein in F-Ep.
In this experiment, no direct effect of LPS upon the mitotic activity of the epitherial cells is recognized. LPS stimulated CH-Fi, produces growth factor and stimulates the mitotic activity of CH-Ep. Such an epithelial-mesenchymal interaction is not recognized between F-Fi and F-Ep.
Judging from the above facts, epithelial-mesenchymal interaction of cholesteatoma, stimulated by LPS and mesenchymal cells, play the major part in this interaction.

Report

(1 results)
  • 1986 Final Research Report Summary
  • Research Products

    (8 results)

All Other

All Publications (8 results)

  • [Publications] 佐野真一: Ear Research Japan. 16. 291-294 (1985)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] 佐野真一: 日本耳鼻咽喉科学会会報. 89. 1496-1497 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] 佐野真一: Ear Research Japan. 17. 163-167 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] 上出洋介: Ear Research Japan. 17. 171-175 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Shinichi Sano: "Ultrastructural study of cholesteatoma" Ear Research Japan. 16. 291-294 (1985)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Shinichi Sano: "Histological study of middle ear cholesteatoma" Journal of Oto-Rhino-Laryngology of Japan. 89. 1496-1497 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Shinichi Sano: "Epithelial-Mesenchymal Interaction of cholesteatoma" Ear Research Japan. 17. 163-167 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Yousuke Kamide: "Novel Epithelial Clonal Strain of Middle ear cholesteatoma" Ear Research Japan. 17. 171-175 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary

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Published: 1987-03-31   Modified: 2016-04-21  

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