| Project/Area Number |
60570832
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Ophthalmology
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| Research Institution | Kyoto University |
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Principal Investigator |
UENO SATOKI Department of Ophthalmology, Faculty of Medicine, 医学部, 助手 (20109010)
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| Co-Investigator(Kenkyū-buntansha) |
HIRATA AKIRA Department of Ophthalmology, Faculty of Medicine, 医学部, 助手 (10181158)
ISHIGOUOKA HITOSHI Department of Ophthalmology, Faculty of Medicine, 医学部, 助手 (80135590)
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| Project Period (FY) |
1985 – 1986
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| Project Status |
Completed (Fiscal Year 1986)
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| Budget Amount *help |
¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1986: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1985: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | Muller Cells / Cytochemistry / Experimental Retinal Degeneration / Diathermy / Laser Photocoagulation / High Voltage Electron Microscope |
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| Research Abstract |
We have found that potassium-independent paranitrophenylphosphatase and glucose 6 phosphatase activities were restricted to the endoplasmic reticulum of retinal glial Muller cells in the guinea pig retina. Utilizing these newly developed cytochemical method, it could be easier to identify Muller cells after experimental retinal diathermy or laser photocoagulation. Muller cells have revealed mainly three different pathological processes corresponding to strength of stimulation. These pathological changes were also observed under high voltage electron microscopy for obtaining three dimensional view. We have also examine the participation of Muller cells in the transitional areas of the retina such as optic nerve-retinal or cilio-retinal region. In both areas, it could be easily revealed that Muller cells play major role in the connection between adjustcent epithelial components. Consequently, this cytochemical method might be useful for examination of Muller cells not only pathologically but also embryologically, cytologically.
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