| Project/Area Number |
60570876
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Functional basic dentistry
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| Research Institution | Josai dental University, Dept. of Oral Microbiology |
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Principal Investigator |
HANAZAWA Shigemasa Josai Dental University, Dept. of Oral Microbiology , Assoc. Prof., 歯学部, 助教授 (60060258)
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| Co-Investigator(Kenkyū-buntansha) |
AMANO Shigeru Josai Dental University, Dept. of Oral Microbiology, 歯学部, 助手 (90167958)
斉藤 貴江子 城西歯科大学, 歯学部, 助手 (10162190)
三好 武仁 城西歯科大学, 歯学部, 助手 (90174226)
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| Project Period (FY) |
1985 – 1987
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| Project Status |
Completed (Fiscal Year 1987)
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| Budget Amount *help |
¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1987: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1986: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1985: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Keywords | Monoclonal antibody / Periodontal disease associated bacteria / 診断学への応用 / B.gingivalis / 特異精製抗原 |
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| Research Abstract |
Bacteroides gingivalis is found in the humoral oral environment and has been isolated in relatively high proportions from inflamed gingival pockets of adult periodontal patients. Several studies have indicated that immunoglobulin G serum antibody titers against B.gingivalis are increased significantly in adult periodontal patients. These studies suggest that B.gingivalis may be associated with the pathogenesis of adult periodontal disease. For clinical studies of adult periodontal disease, it is important to rapidly detect and identify B.gingivalis in the oral cavity. Specific and highly sensitive markers of B.gingivalis would be of great use to establishe simple methods for clinical investigation. We have established a established cell line that produces B.gingivalis-specific monoclonal antibody by hybridization technology. Purpose of this study was to evaluate for application of the monoclonal antibody for diagnosis of periodontal disease. An antigen recognized by the monoclonal antibody specific for B.gingivalis was purified by immunoadsorbet column. The purified antigen was homogeneous as jodged by SDS-PAGE and silver staining, and the pattern of wester blotting. The antigen molecule exhibited an apparent molcular weight of about 62,000. A significant positive correlation was observed between seru IgG antibody titer against the specific antigen. However,B.gingivalis was not isolated in significant high frequency from subgingival plaques of adult periodontal patients. Therefore, it is very important to elucidate this discrepancy in feature.
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