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A basic study on the development of diagnostic and treatment for periodontal diseases using monoclonal antibodies against human periodonopathic bacteria.

Research Project

Project/Area Number 60570879
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Functional basic dentistry
Research InstitutionTokyo Dental College

Principal Investigator

NAITO Yuko  Department of Microbiology, Tokyo Dental College, 歯学部, 助手 (00147258)

Co-Investigator(Kenkyū-buntansha) 小野 美千代  東京歯科大学, 微生物, 助手 (60167327)
KATO Tetsuo  Department of Microbiology, Tokyo Dental College, 微生物, 助手 (00159253)
OKUDA Katsuji  Department of Microbiology, Tokyo Dental College, 微生物, 助教授 (40085741)
ONO Michiyo  Department of Microbiology, Tokyo Dental College (40112669)
Project Period (FY) 1985 – 1986
Project Status Completed (Fiscal Year 1986)
Budget Amount *help
¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1986: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1985: ¥1,400,000 (Direct Cost: ¥1,400,000)
KeywordsIgG response / B. gingivalis / Adult periodontitis / Monoclonal antibody / Hemagglutinin / LPS / 診断 / IgG抗体
Research Abstract

Bacteroides gingivalis has been recognized as causing adult periodontitis. Antibody elevations in adult patients with periodontitis in response to the concentration of the organisms have been reported in our labolatory. The present research was done to determine which surface antigens was useful in detecting serum antibody to the microorganisms by immunoblotting analysis with monoclonal antibodies. Almost all sera obtained from the patients reacted with sonicate antigen, glass-beads extracted antige, and attachment factor(hemagglutinin) of the cells. Most sera from adult periodontitis sera reacted strongly with hemagglutinin, but a considerable variation in antibody levels against lipopolysaccharide(LPS) and capsular antigen was found. The specific IgG level to B. gingivalis was determined by a micro ELISA. A statistically positive correlation was found between IgG levels against the antigens and the degree of alveolar bone loss. On the other hand, no significant correlation was found between the antibody levels against the antigens and pocket depth. The sera obtained from patients reacted with hemagglutinin were confirmed by the immunoblotting tests. Sera from healthy individuals did not react with these antigens.
It is interesting that most sera from patients with adult periodontitis possessed high antibody levels to hemagglutinin. This result indicates that addhessive surface structures such as hemagglutinin participate as a first step in B. gingivalis colonization and antigenic stimulation. The present findings emphasize the use of hemagglutinin to detect antibodies against B.gingivalis during the initial stages of adult periodontitis.

Report

(1 results)
  • 1986 Final Research Report Summary
  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] Okuda,K;Kato,T;Shiozu,J;Takazoe,I;Nakamura,T: International Journal of Systematic Bacteriology. 35. 438-442 (1985)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Okuda,K;Ohta,K;Kato,T.Takazoe,I;Slots,J.: Journal of Clinical Microbiology. 24. 89-95 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Okuda,K;Kato,T;Naito,Y;Takazoe,I.: Journal of Clinical Microbiology. 24. 846-848 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Okuda,K;Kato,T;Naito,Y;Ono,M;Kikuchi,Y;Takazoe,I.: Journal of Dental Research. 65. 1024-1027 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Naito,Y;Okuda,K;Kato,T;Takazoe,I: Infection and Immunity. 50. 231-235 (1985)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Okuda,K;Yamamoto,A;Naito,Y;Takazoe,I;Slots,J;Genco,R.J.: Infection and Immunity. 54. 659-665 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Takazoe,I;Okuda,K.: "Pathogenic of black-pigmented Bacteroides in periodontal disease.Proceedings of 2nd International Symposium in Anaerobes." Japan Association for Anaerobic Infection for Research, 205-217 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Takazoe,I;Okuda,K.: "Immunology of periodontal disease.Proceedings in 【VIII】 International Conference of Labeled Antibodies." Tokyo University Publisher,

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Naito,Y., OkudaK., Kato,T. and Takazoe,I.: "Monoclonal antibodies against surface antigens of Bacteroides gingivalis." Infection and Immunity. 50. 231-235 (1985)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Okuda,K.,Kato,T., Naito,Y. and Takazoe,I.: "Precipitating antibody against lipopolysaccharide of Haemophilus actinomycetemcomitans." J.Clin. Microbiol.24. 846-848 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Okuda,K., Kato,T., Naito,Y., Ono,M., Kikuchi,Y. and Takazoe,I.: "Susceptibility of Bacteroides gingivalis to bactericidal activity of human serum." J. Dent. Res.65. 1024-1027 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Okuda,K., Yamamoto,A., Naito,Y., Takazoe,I., Slots,J. and Genco,R.J.: "Purification and properties of hemagglutinin from culture supernatant of Bacteroides gingivalis." 54. 659-665 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Naito,Y., Okuda,K. and Takazoe,I.: "Detection of specific antibody in adult human periodontitis sera to surface antigens of Bacteroides gingivalis." Infection and Immunity. 55. 832-834 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Naito,Y. and Okuda,k.: Saikon publisher. Monoclonal antibody to bacterial surface antigens. In " Monoclonal Antibody" ( in Japanese ), 26-37 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary

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Published: 1987-03-31   Modified: 2016-04-21  

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