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Studies on the oral biological functions of low moleculat salivary peprides

Research Project

Project/Area Number 60570882
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Functional basic dentistry
Research InstitutionThe Nippon Dental University

Principal Investigator

KAZUO Sanada  The Nippon Dental Universiy, School fo Dentistry at Niigata, 新潟歯学部, 教授 (70060423)

Co-Investigator(Kenkyū-buntansha) EIIHI Saitoh  The Nippon Dental University, School of Dentistry at Niigata, 新潟歯学部, 講師 (40120662)
HIROMI Shimomura  The Nippon Dental University, School of Dentistry at Niigata, 新潟歯学部, 助教授 (40139259)
SATOKO Isemura  The Nippon Dental University, School of Dentistry at Niigata, 新潟歯学部, 助教授 (10112963)
Project Period (FY) 1985 – 1987
Project Status Completed (Fiscal Year 1987)
Budget Amount *help
¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1987: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1986: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1985: ¥1,000,000 (Direct Cost: ¥1,000,000)
KeywordsSaliva / peptide / Fental plaque / Organic acid production / 炭酸カルシウム沈澱形成阻害 / 唾液ペプチド / 歯苔の有機酸生成
Research Abstract

To find out the oral biological functions of solivary low molecular peptides, their effects on inhibition of calcium-carbonate precipitaion, on organic acid production of dental plaque microorganisms, and on degradation of orgthodontic rubber elasticus were studied. The results were as follows. 1. The inhibitory effect of salivary peprides on calcium carbouate precipitation were observed. The effects of salivary pepride fraction on the rate of precipitation of CaCO3 from a CaCO3-supersafurated solution were examined by recording the absorgance at 570nm and the pH, when 20M CaCL2 was added to 20mM NaHCO_3, in the presence ot absence of peptides. The low molecular peprides(mw = 3000, 1000 - 2000) supressed CaCO_3 nuclaetion and exhibited inhibitory effects on CaCO precipitation under the experimental conditions. These peptides may be of biological significance in maintaining homeostasis of the buffering system of saliva, which is mainly composed of bicarbonate, and in preventing the fromation of stones consiting of CaCO3 in the salivary ducts. 2. The inhibirtory effect of salivary peptides on acetic acid production under the anaerobic condition by plaque microorgamisms were observed. 3. Cysteind proteinase inhibitors in human saliva(Cystatin-SA,-SN) were inhibited the production of lactic acid acetic acid in the BHI-broth contaning dental plaque. 4. The effects of saliva on degradation of elastics were tested. the degradation of elastics was enhanced by prolonged immersion in the solutions containing urea, uric acid, amino acids, glutathion, and vitamins or imcubated whole salivva (37゜C, 6-18hr), respectively. It was conceivable that salivary peprides affect on the degradation of elestaics.

Report

(2 results)
  • 1987 Final Research Report Summary
  • 1986 Annual Research Report
  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Eiichi,Saitoh: Archives of Oral Biology. 30. 641-643 (1985)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 鹿島俊彦: 歯学. 73. 1741-1753 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 森谷健之: 歯学. 73. 1767-1779 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 真田一男: 歯学. 74. 16-22 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Satoko,Isemura: The Journal of Biochemistry. 102. 693-704 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] eiichi, Saitoh: "Inhibition of calcium-carbonate precipitation by hyman salivary prolinr-rich phosphoproteins" Archives of Oral Biology. 30. 641-643 (1985)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Toshihiko, Kashima: "Inhibitory effect to human saliva on precipitation of calcium carbonate" SHIGAKU. 73. 1741-1753 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Takeyuki, Moriya: "Inhibitory effect of hyman parotid salivary proteins on precipitation of calcium carbonate" SHIGAKU. 73. 1767-1779 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Kazuo, Sanada: "Effect of salivary components on degradation of orthodontic rubber elastice" SHIGAKU. 74. 16-22 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Satoko, Isemura: "Characterization and amino acid sequence of a new acidic cysteine proteinase inhibitor (Cystatin SA) structurally closely related to Cystatin S, from hyman whole saliva" The Journal of Biochemistry. 102. 693-704 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 森谷健之,寺田あかね,斉藤英一: 歯学. 73. 1767-1779 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] 鹿島俊彦,寺田あかね,真田一男: 歯学. 73. 1741-1753 (1986)

    • Related Report
      1986 Annual Research Report

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Published: 1987-03-31   Modified: 2016-04-21  

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