| Project/Area Number |
60570923
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
外科・放射線系歯学
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| Research Institution | Niigata University |
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Principal Investigator |
KAWASAKI Tateharu School of Dent.,Niigata Uni. , Associate Proffessor, 歯学部, 助教授 (70009653)
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| Co-Investigator(Kenkyū-buntansha) |
KIMURA Yutaka School of Dent., Niigata Uni., Assistant, 歯学部, 助手 (30177935)
SHINGAKI Susumu University Hospital(Dental), School of Dent., Niigata Uni. , Lecturer, 歯学部附属病院, 講師 (30134943)
NAKAJIMA Tamio School of Dent.,Niigata Uni. , Proffessor, 歯学部, 教授 (10014010)
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| Project Period (FY) |
1985 – 1986
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| Project Status |
Completed (Fiscal Year 1986)
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| Budget Amount *help |
¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1986: ¥500,000 (Direct Cost: ¥500,000)
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| Keywords | salivary gland tumor / cytology / nuclear DNA contents / flow cytometry / cell cycle / cell cycle |
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| Research Abstract |
Flow cytometric DNA measurements were performed on 6 salivary gland tumors, consisted of 1 benign pleomorphic adenoma, 2 adenoid cystic carcinomas, 1 acinic cell carcinoma, 1 malignant salivary gland tumor and 1 adenocarcinoma. Non-neoplastic controls consisted of 12 differrent specimens obtained from submandibular salivary glands, lymphonodes, and oral mucosal epitheliums. DNA ploidy and cell cycle distributions were calculated from DNA histograms and compared to cytologic data.
Only 2 tumors ( 1 malignant salivary tumor and 1 adenocarcinoma) were clearly diagnosed as malignancy from their cytologic smears. But in the other 3 malignant tumors, cytologic diagnosis was very difficalt due to lack of cytologic malignant characteristics.
In the DNA analysis, 4 of the 6 tumors (1 benign pleomorphic adenoma, 2 adnoid cystic carcinomas and 1 acinic cell carcinoma) showed 'nonmal' DNA diploid histograms such as seen in non-neoplastic control tissues. The DNA histogram of malignant salivary tumor had a diploid pattern with a significant increase in the tetraploid peak. The specimen of adenocarcinoma contained two types of tumor cells : a DNA diploid peak and a distinct DNA aneuploid peak. This tumor seemed to be a multiclonal type. The percentage of cells in S-phase (S-index) for all the diploid tumors were lower than for non-neoplastic controls. This finding indicated that the proliferantive activity of these tumors were lower than normal tissues. The S-index for the aneuploid tumor was simillar to the mean value for the non-neoplastic tissues.
DNA analysis can offer distinctive tumor biological properties such as multiclonality and proliferent activity, and may yield clinically valuable information.
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