| Project/Area Number |
60570924
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
外科・放射線系歯学
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| Research Institution | Nagoya University |
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Principal Investigator |
UEDA Minoru Department of Oral Surgery,School of Medicine,Nagoya University, 医学部, 助手 (00151803)
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| Co-Investigator(Kenkyū-buntansha) |
EBATA Koji Department of Oral Surgery, School of Medicine, Nagoya University, 医学部, 医員 (10203647)
嶋 隆義 名古屋大学, 医学部, 医員
SHIMA Takayoshi Department of Oral Surgery, School of Medicine, Nagoya University
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| Project Period (FY) |
1985 – 1986
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| Project Status |
Completed (Fiscal Year 1986)
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| Budget Amount *help |
¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1986: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1985: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | Tissue culture / Artificial Mucosa / Intraoral reconstruction / 粘膜移植 |
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| Research Abstract |
1) Constitution of Bioartificial Mucosa : To prepare a bioartificial mucosa fibroblasts were isolated from a rabbit oral mucosa, cultured and harvested in collagen gel, 6 x 10 cells/dish for at least 10 days. The culture condition was 37゜C, 5% <CO_2> , 15%MEM+fetal calf serum.
2) Transplantation of Bioartificial Mucosa : The bioartificial mucosa was transplanted on the skin defect prepared on the dorsum of the same animal. The bioartificial mucosa was too weak to be sutured. So bioadhesive was used to fix the graft. The most important point in the manner of transplantation was to prevent the infection and to be dry. To prevent these trouble, we emplied the ointment containing the antibiotics. The graft was vasculized 7 days after transplantation and survived completely. No imunological rejection was observed.
3) Microstructure of Bioartificial Mucosa : The microstructure of the bioartificial mucosa was studied by transparent erectoron microscop especially basal lamina. Four weeks after culturing hemi-desmosome was observed in the border between epithelial cells and mesenchymal cells. However, complete structure of basal lamina was no seen at that time.
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