A study for mecanism of polyclonal B cell activation in patient with cancer. (I)

Research Project

Project/Area Number	60570948
Research Category	Grant-in-Aid for General Scientific Research (C)
Allocation Type	Single-year Grants
Research Field	外科・放射線系歯学
Research Institution	Kanagawa Dental College
Principal Investigator	YAMAGUCHI Misao Kanagawa Dental College, the faculty of Dental Science, 歯学部, 講師 (80104438)
Co-Investigator(Kenkyū-buntansha)	久米川一浩神奈川歯科大学, 歯学部, 助手 (50170064) OHASHI Mikio Kanagawa Dental College, thw faculty of Dental Science, 歯学部, 助手 (00160599) SASAKURA Yuuichi Kanagawa Dental College, the faculty of Dental Science, 歯学部, 助手 (80121002) KUMEGAWA Kazuhiro Kanagawa Dental College, the faculty of Dental Science
Project Period (FY)	1985 – 1986
Project Status	Completed (Fiscal Year 1986)
Budget Amount *help	¥400,000 (Direct Cost: ¥400,000) Fiscal Year 1986: ¥400,000 (Direct Cost: ¥400,000)
Keywords	Actinomyces viscosus / Human peripheral blood lymphocytes / Flow cytometer / TCA cycle / Succinate metabolism / Immunoglobulin synthesis / General anesthesia
Research Abstract	1. The supernatant of sonicated Actinomyces viscosus (AVsup) were examind for it's mitogenecity to human peripheral blood lymphocytes (PBL).: AVsup induced a low increase of DNA synthesis on T cell and B cell fraction, but a higher increase on unfractionated lymphocytes (UNF). AVsup did not induced of OKT3(+), OKT4(+), OKT8(+) cell population. However, it increased OKDR(+) cell population included activated T cell, 70% of B cells, 55% of monocytes, but only monocytes were excluded by appropriate gating on the cytogram of the flow cytometer, although it faild to increased OKB7(+) cell population. So the increasing of OKDR antigen positive cell population was considered not as B cells but activated T cell. 2. Human PBL were cultured with succinate 1,4- <^(14)C> , which is a TCA cycle intermediated metabolic substance, in the stimulation with pokeweed mitogen (PWM). It was recognized that <^(14)C> was taken into the lymphocytes, utillized for cell metabolism and finally synthesized into im … More munoglobulins.: (1) <^(14)C> concentration in the UNF kept at low level for 1-7 days when cultured without PWM. However the stimlating with PWM increased the level by 3 times in the first day, and then they decleased with day. (2) <^(14)C> concentration in the macromolecucular substances detected in the PWM-stimulated culture sup increased with time from the third day on. The maximum value of <^(14)C> in the macromolecular substances was 0.4% of that initially added. (3) Immunoglobulins (IgG,A and M) containing <^(14)C> were detected in the sup after 7 days culture with PWM. They showed 5.6% of <^(14)C> which was detected in the macromolecular substances in the sup. The order of <^(14)C> content was B<UNF<T+B fraction. 3. Infuluence of general anesthesia on the percentage of lymphocyte subset in PBL. Lymphocyte membrane markers were analysed with flow cytometry. The results demonstrated that the positive percentage of CD3, CD4, CD20 and the ratio of CD4/8 were found to be significantly lower, and that of CD8 were found to be significantly higher during anestesia than pre and postanesthesia. Less