| Project/Area Number |
60571027
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Physical pharmacy
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| Research Institution | Showa University |
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Principal Investigator |
MAEDA Masako School of Pharmaceutical Sciences, Showa University, 薬学部, 助教授 (00053869)
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| Co-Investigator(Kenkyū-buntansha) |
ARAKAWA Hidetoshi School of Pharmaceutical Sciences, Showa University, 薬学部, 助手 (70129807)
TSUJI Akio School of Pharmaceutical Sciences, Showa University, 薬学部, 教授 (80053784)
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| Project Period (FY) |
1985 – 1986
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| Project Status |
Completed (Fiscal Year 1986)
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| Budget Amount *help |
¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1986: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1985: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | Lucigenin / NAD(P)H / Biological Compounds / Chemiluminescence Reaction / Chemiluminescence / Chemiluminescence HPLC / デヒドロゲナーゼ / 酵素免疫測定法 / グルコース / イソルミノール / m-POD / 1-MPMS / ATP |
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| Research Abstract |
Chemiluminescence assay is attractive analytical method in the field of clinical chemistry, because of their sensitivity, rapidity, and wide dynamic range. The chemiluminescent agents most commonly used for analytical purposes are luminol and its derivatives and lucigenin. Peroxyoxalate-sensitized chemiluminescence is also applied to determination of <H_2> <O_2> and fluorescent compounds. We also developed new chemiluminescent reaction for NAD(P)H by using l-methoxyphenazinium methosulfate-isoluminol-m-POD.
These chemiluminescent reaction were applied to determination of biological substances such as steroids, bile acids, thyroxine, glucose and various enzyme activity in biological fluids by using high performance liquid chromatographic, flow injection analysis or enzyme immunoassay techniques.
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