Studies on the continuous production of natural medicines by the immobilized plant cell bioreactors.
| Project/Area Number |
60571055
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Biological pharmacy
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| Research Institution | Kitasato University |
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Principal Investigator |
FURUYA Tsutomu Pharmaceutical Sciences of Kitasato University, Professor, 薬学部, 教授 (10050345)
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| Co-Investigator(Kenkyū-buntansha) |
ORIHARA Yutaka Pharmaceutical Sciences of Kitasato University, Assistant Professor, 薬学部, 助手 (30137905)
AYABE Shinichi Pharmaceutical Sciences of Kitasato University, Assistant Professor, 薬学部, 助手 (40050679)
YOSHIKAWA Takafumi Pharmaceutical Sciences of Kitasato University, Associate Professor, 薬学部, 助教授 (80050540)
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| Project Period (FY) |
1985 – 1986
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| Project Status |
Completed (Fiscal Year 1986)
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| Budget Amount *help |
¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1986: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1985: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | Immobilized plant cell / Production of useful compounds / Bioreactor / Continuous production of natural products / Coffea arabica / Panax ginseng / Glycyrrhiza echinata |
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| Research Abstract |
The immobilized microbial cell systems have rapidly developed during recent years. Practically, these systems have been applied to the industrial production of useful compounds such as foodstuff additives and drugs. However, there are few papers concerning the application of immobilized plant cells to the production of useful natural compounds. So, in this project we investigated first the method to immobilize a plant cell and next the application of the immobilized plant cell to various reactions concerning saponin, flavonoid and alkaloids.
A variety of matrices to immobilize microbial cell have been employed, but calcium alginate was especially favorable to plant cells because of several operational advantages, i.e. mild conditions and high retention of cell viability. Intact plant cultured cells were entrapped in calcium alginate beads under sterile conditions. The immobilized cells slightly increased in the beads when they were cultured in a flask supplemented with the growing medium in a rotary shaker. Using these immobilized living plant cells, this project was carried out to resolve the next three problems.
(1) The application to the production of useful compounds. (2) Continuous production of intracellular compounds by permeabilization. (3) Development of continuous production system using bioreactor. As the result, we carried out first the production of caffeine and theobromine by the immobilized Coffea arabica cells. Next, saponin of Panax ginseng and flavonoids of Glycyrrhiza echinata mutant cells were excreted by DMSO(dimethylsulfoxide) treatment with no effect on the cell viability from the cultured roots and the immobilized cells, respectively. Finally, we succeeded in the continuous production of berberine and its related compounds directly using Coptis japonica cultured root for the revised bioreactor.
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Report
(1 results)
Research Products
(6 results)
