• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Studies on the relation between the biological function and the dynamic properties of rat liver plasma and microsomal membranes

Research Project

Project/Area Number 60571057
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Biological pharmacy
Research InstitutionShowa University

Principal Investigator

UTSUMI Hideo  Associate-Professor, School of Pharmaceutical Sciences, 薬学部, 助教授 (20101694)

Co-Investigator(Kenkyū-buntansha) YOSHIDA Takemi  Associate-Professor, School of Pharmaceutical Sciences, 薬学部毒物学, 助教授 (20138415)
Project Period (FY) 1985 – 1986
Project Status Completed (Fiscal Year 1986)
Budget Amount *help
¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1986: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1985: ¥1,300,000 (Direct Cost: ¥1,300,000)
Keywordsrat liver microsomes / spin-label / ESR / biomembrane / phenobarbirtal / cytochrome P-450 / フェノバルビタール / 生体膜
Research Abstract

To elucidate the relation between the cellular function and the dynamic properties of biomembranes, we studied the membrane fluidity and nitroxide reduction activity of rat liver microsomes with spin-label technique.
1. Four spin-labeled stearic acids were used as both probes for membrane fluidity and substrates for nitroxide reduction by NADPH cytochrome P-450 reductase - cytochrome P-450 system. Active center of nitroxide reduction of microsomes was found to locate around 7-12 carbon position of stearic acid in membranes. The nitroxide reduction was induced by the administration of phenobarbital but not by 3-methylcholanthrene. However, the membrane fluidity of liver microsomes increased by the administration of phenobarbital, polychlorinated biphenyls or 3-methylcholanthrene.
2. Oral administration of C <Cl_4> , CH <Cl_3> , CH <Br_3> and CH <Br_2> Cl changed membrane structure and nitroxide reduction activity of rat liver microsomes. ESR spectrum of methyl ester of spin-labeled steari … More c acid in microsomes showed the presence of non-bilayer and bilayer phases in membranes. The transfer rate of the spin probes between two phases was changed by the oral administration of these compounds. Nitroxide reduction rate also decreased by the treatment. Among these compounds, C <Cl_4> gave the most influence on the membrane fluidity and nitroxide reduction.
3. In vitro treatment of C <Cl_4> and CH <Br_3> to rat liver microsomes caused lipid peroxidation of microsomal membranes in addition to decrease of cytochrome P-450 contents, arylesterase and demethylase activity when NADPH generating system was added, but not in the absence of the system. These compounds increased the membrane fluidity of liver microsomes, while the metabolites of the compounds decreased. Nitroxide reduction activity of microsomes also decreased by the compounds themselve, but much decrease was observed in the presence of NADPH generating system.
4. Long-term administration of phenobarbital to rat caused significant decrease of the activity in drug metabolizing enzymes. Less

Report

(1 results)
  • 1986 Final Research Report Summary
  • Research Products

    (8 results)

All Other

All Publications (8 results)

  • [Publications] Hideo Utsumi;Jun-Ichiro Murayama;Akira Hamada: Biochem.Pharmacol.34. 57-63 (1985)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Hideo Utsumi;Jun-Ichiro Murayama;Kayoko Fukuda;Akira Hamada: 31. P-29- (1985)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Hideo Utsumi, Jun-Ichiro Murayama and Akira Hamada: "Structural changes of rat liver microsomal membranes induced by the oral administration of carbon tetrachloride --31P-NMR and spin-label stdies--" Biochem. Pharmacol.34. (1985)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Hideo Utsumi, Jun-Ichiro Murayama, Kayoko Fukuda and Akira Hamada: "Effect of organic halogen compounds on the structure and reductase activity of rat liver microsomal membranes (II)" Eisei Kagaku. 31. (1985)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Hideo Utsumi, Akira Shimakura and Akira Hamada: "Location of active center of nitroxide reduction in rat liver microsomes and its membrane fluidity - The effect of typical inducers" SUBMITTED.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Jun-Ichiro Murayama, Hideo Utsumi and Akira Hamada: "Effect of CH <Br_3> and CH <Br_2> Cl on rat liver microsomal membranes by oral administration. Spin-label studies"

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Norimitsu Kurata, Takemi Yoshida and Yukio Kuroiwa: "Long-term changes in drug-metabolizing enzymes and heme metabolizing enzymes during chronic treatment of rats with phenobarbital"

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Kayoko Fukuda, Akira Shimakura, Hideo Utsumi and Akira Hamada: Structural and enzymatic changes of rat liver microsomal membranes by the hepatotoxic actions of halogenomethanes,

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary

URL: 

Published: 1987-03-31   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi