Research on a peptide differentiation factor for myeloid leukemia cells

Research Project

Project/Area Number	60571058
Research Category	Grant-in-Aid for General Scientific Research (C)
Allocation Type	Single-year Grants
Research Field	Biological pharmacy
Research Institution	Showa University
Principal Investigator	NAKAYA Kazuyasu Sch. Pharm. Sci., Showa University, Associate Professor, 薬学部, 助教授 (40053855)
Project Period (FY)	1985 – 1986
Project Status	Completed (Fiscal Year 1986)
Budget Amount *help	¥1,400,000 (Direct Cost: ¥1,400,000) Fiscal Year 1986: ¥500,000 (Direct Cost: ¥500,000) Fiscal Year 1985: ¥900,000 (Direct Cost: ¥900,000)
Keywords	Differentiation / Differentiation factor / Peptide / Leukemia cells / Peptide differentiation factor
Research Abstract	Naturally occurring differentiation factors are expected to be useful in leukemia therapy because of their low nonspecific toxicity to the host. we found cells spontaneously differentiated from mouse leukemia M1 cells were producing a low molecular weight factor that induces differentiation in M1 cells. In this study, we established a method to purify this factor. The procedure for purification includes gel filtration on Sephadex G-15, anionic exchange chromatography, thin-layer chromatography, reverse-phase HPLC on a C18 hydrophobic support. and HPLC gel filtration. Amino acid analysis and the color developed by the reaction of Rydon and Smith idicate that the factor is a peptide composed of 12 amino acids. The <NH_2> -terminal of the factor is blocked. M1 cells were induced to differentiatte into macrophage-like cells by the purified fctor. The purified factor at the concentration of 5 x <10-^7> g/ml also inhibits the growth of human leukemia HL-60 cells.