| Co-Investigator(Kenkyū-buntansha) |
NAMIKAWA MACHIKO Kobe-Gakuin University, Pharmaceutical Science,Associate Research, 薬学部, 助手 (60155984)
HAMA TAKAO Kobe-Gakuin University,Pharmaceutical Science, Professor, 薬学部, 教授 (10068215)
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| Budget Amount *help |
¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1986: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1985: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Research Abstract |
every cell nucleus contains the complete genome as established in the fertilized egg. In molecular terms, the DNAs of all differentiated cells are identical. Development, then, involves the differential expression of specific genes at specific place and times. In other word, the different cell types expressing different genes. Only a small percentage of the genome is being expressed in each cell, and a portion of the RNA synthesized is specific for that cell type. The unused genes in differentiated cells are not destroyed or mutated, and they retain the potential for being expressed. How can these phenomena explain?
We examine the effect of Bredinin which has teratogenic effects on embryonic development. The effect of nuclear transplantation into the fertilized egg on the following development of the microinjected eggs in vitro or in vivo. The same time, we studied the teratogenesis of these embryo.
(Results)
Bredinin inhibited the embryonic development in vitro, that is embryonic cleavage, hatching, attaching the embryos to culture substratum and trophoblast outgrowth. Especially, it inhibited morula development into blastocyst (i.e.,the first step of differentiation) most effectively. On the other hand, it didn't inhibit the ICM formation step.
In nuclear transplanting experiments, the triploid embryo which were produced by syngeneic nuclear transplantation developed into blastocyst stage, over 50%. Even in foreign nuclear transplantation (chinese hamster), the rate of development decreased but not zero.
These results suggest that the possibility of therapeutics of hereditary disease.
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