• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

ENZYME-LINKED IMMUNOSORBENT ASSAY(ELISA) FOR DETECTION OF MUMPS IgM ANTIBODY

Research Project

Project/Area Number 60571079
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 医学一般
Research InstitutionNATIONAL INSTITUTE OF HEALTH

Principal Investigator

SAKATA HIROKO  CHIEF RESEARCHER,DEPART. MEASLES VIRUS, NIH, その他, 研究員 (70175363)

Co-Investigator(Kenkyū-buntansha) YAMADA AKIO  RESEARCHER, DEPART. MEASLES VIRUS, NIH, 麻疹ウイルス部, 研究員 (50150876)
TURUDOME MASATO  ASSISTANT, DEPART. MEDICINE, MIE UNIV., 医学部, 助手 (50159042)
ITO YASUHIKO  PROFESSOR, DEPART. MEDICINE, MIE UNIV., 医学部, 教授 (00022872)
SUGIURA AKIRA  DIRECTOR, DEPART. MEASLES VIRUS, NIH, 麻疹ウイルス部, 部長 (80077172)
Project Period (FY) 1985 – 1986
Project Status Completed (Fiscal Year 1986)
Budget Amount *help
¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1986: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1985: ¥900,000 (Direct Cost: ¥900,000)
KeywordsMumps / IgM capture ELISA / 血清学的診断
Research Abstract

Serological disgnosis of viral infection is done by the demonstration of a significant increase of antibody during the period from acute to convalscent phases. Bleeding of patients at appropriate times is, however, not always possible and, in many instances, including mumps, only one serum specimen is available. Domonstration of IgM antibody provides the evidence for a recent infection with mumps virus. IgM antibody has usually been determined after separation of IgM fraction from a test serum by centrifugation through sucrose gradient, but the method is not easy for a routine serodiagnosis. We tried to develop a less sophisticated and less time-consuming procedure for determination of IgM antibody against mumps virus. We have developed the method in which IgM of a test serum is captured by anti-human IgM antibody attached to the solid phase. Purified mumps virus and peroxidase-conjugated monoclonal antibody specific to mumps virus NP protein then followed in succession. The color generated by the peroxidase in reaction with the substrate mixture was determined spectrophotometrically: In 24 cases of clinical mumps, the diagnosis of which had been confirmed by a significant rise in antibody titer in IgG-ELISA, IgM antibody was positive in 6 out of 9 sera taken on the first day of illness, in 8 out of 9 on day 2, and in all of 24 on day 5 and thereafter. A follow-up study with 25 cases showed that IgM antibody persisted for as long as 3 months and in one case up to 5 months. These results showed that the method is useful for the diagnosis of recent mumps virus infection.

Report

(1 results)
  • 1986 Final Research Report Summary
  • Research Products

    (7 results)

All Other

All Publications (7 results)

  • [Publications] H.Sakata;et.al.: Joumal of Virological Methods. 12. 303-311 (1985)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] M.Tsurudome;et.al.: Journal of General Virology. 67. 2259-2265 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] 野村恭也他: 耳鼻咽喉臨床.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] H.Yoshikawa: J.Pestic.Sci.11. 631-633 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Y.J.Ahn: J.Pestic.Sci.12. 71-77 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] H. SAKATA, M. TSURUDOME, H. HISIYAMA, Y. ITO AND A. SUGIURA: "ENZYME-LINKED IMMUNOSORBENT ASSAY(ELISA) FOR MUMPS IgM ANTIBODY: COMPARISON OF IgM CAPTURE AND INDIRECT IgM ASSAY" JOURNAL OF VIROLOGICAL METHODS. 12. 303-311 (1985)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] M.TSURUDOME, M. HISIYAMA, Y. ITO AND A. YAMADA: "MONOCLONAL ANTIBODIES AGAINST THE GLYCOPROTEINS OF MUMPS VIRUS: FUSION INHIBITION BY ANTI-HN MONOCLONAL ANTIBODY" JOURNAL OF GENERAL VIROLOGY. 67. 2259-2265 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary

URL: 

Published: 1987-03-31   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi