| Project/Area Number |
60571098
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
応用薬理学・医療系薬学
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| Research Institution | Central Institute for Experimental Animals |
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Principal Investigator |
ANDO Kiyoshi Dept. Psychopharmacology, Central Institute for Experimental Animals, その他, 研究員 (90072404)
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| Co-Investigator(Kenkyū-buntansha) |
MIYATA Hisatsugu Preclinical Research Lab., Central Institute for Experimental Animals, 前臨床医学研究所, 特別研究員
HIRONAKA Naoyuki Dept. Psychopharmacology, Central Institute for Experimental Animals, 精神薬理部, 研究員 (60173291)
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| Project Period (FY) |
1985 – 1986
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| Project Status |
Completed (Fiscal Year 1986)
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| Budget Amount *help |
¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1986: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1985: ¥800,000 (Direct Cost: ¥800,000)
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| Keywords | Rat / Delayed discrimination / Y maze / Operant chamber / Scopolamine / Physostigmine / AF64A |
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| Research Abstract |
Delayed discrimination behavior was established in a Y-maze situation and in 2-lever operant chamber situations in rats where a discriminative stimulus light either on the right side or the left side was presented for a brief period. Running responses or responses on the lever to the lighted side were reinforced with food pellets. After establishment of the above behavior, the percentage of correct choice responses to the discriminative stimulus side was observed with various delay times (0-32 sec) after extinguishing the discriminative light. More than 80% was attained just after termination of the discriminative light (delay = 0 sec) while the longer the delay time elapsed, the lower the percentage. In comparison with saline control, scopolamine at 0.06 mg/kg, sc, physostigmine at 0.06 mg/kg, sc, arecoline at 1 mg/kg, sc, and diazepam at 1 mg/kg, sc, decreased the percentage at various delay times including 0 sec. AF-64A at 6 nmol per rat was given intraventricularly to the rats which had showed established delayed discrimination responses in Y-maze situation. The responses were disrupted for more than 2 weeks after the administration while the responses in rats administered saline intraventriculary were not disrupted. Testing the improving effects of drugs on the disrupted responses by AF-64A was remained for further study. In conclusion, the present behavioral methods may be useful for evaluating drug effects on functions relating memory processes in animals and AF-64A may also be useful for studying memory related dysfunctioning in relation with cholinergic nervous system.
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