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Study on Dertermination of Vitamin C with Biosensor

Research Project

Project/Area Number 60580051
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 家政学
Research InstitutionOchanomizu University

Principal Investigator

ARAKAWA Nobuhiko  Ochanomizu University , Professor, 家政学部, 教授 (40017223)

Project Period (FY) 1985 – 1986
Project Status Completed (Fiscal Year 1986)
Budget Amount *help
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1986: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1985: ¥1,400,000 (Direct Cost: ¥1,400,000)
KeywordsVitamin C / Biosensor / アスコルビン酸酸化酵素
Research Abstract

Generally, 2, 4 dinitrophenyl hydrazine (DNP) method is employed as a standard method for the determination of vitamin C in foods. However, the operation procedures of this method are rather complicated and time consuming, so a more convenient and rapid determination method is necessary. It is well known that ascorbic acid oxidase specifically catalyzes the oxidation reaction of ascorbic acid (AsA) to dehydroascorbic acid (DAsA). In this study, we used a biosensor and determined AsA by the estimation of the decreasing amount of dissolved oxygen (DO) in the solution consumed during the reaction. We established a method for the determination of total vitamin C by using dithiothreitol as reducing agent for the DAsA reduction. The optimum analysis conditions for the determination of AsA and reduction conditions of DAsA were established by estimating the consumption of DO during reaction. The two determination methods, the standard method and the sensitive one for high and low AsA concentration samples respectively, were established. A linear relationship was found between the DO uptake and AsA DAsA concentrations. AsA and DAsA concentrations were twice the DO uptake, thus this method makes it possible to determine AsA and DAsA without using a calibration curve. It was found that the determination level of AsA and DAsA as low as 0.01mM is possible using this biosensor system. The values of AsA contents in the juice obtained using the present method have a good comparability to those obtained by DNP and HPLC method. The procedure for this analytical method was quite simple and applicable for small amount samples. Due to the specificity of the enzyme used, it was possible to determine vitamin C accurately. This method is applicable for a rapid determination of total vitamin C contents in foods.

Report

(1 results)
  • 1986 Final Research Report Summary
  • Research Products

    (2 results)

All Other

All Publications (2 results)

  • [Publications] 荒川信彦,道家晶子,倉田忠男: 日本食品工業学会誌.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Nobuhiko Arakawa, Shouko Douke and Tadao Kurata: "Determination of Ascorbic acid with Oxygen Electrode" Journal of the Japnese Society for Food Science and Technology.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary

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Published: 1987-03-31   Modified: 2016-04-21  

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