| Budget Amount *help |
¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1986: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1985: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Research Abstract |
The isoform of skeletal muscle alpha-actinin present during chick embryogenesis were analyzed. Chicken embryonic muscle at ages 8-15 days contain an embryo-specfic isoform of alpha- actinin. Developmental sequence of alpha-actinin isoforms expression was also studied. In extracts of skeletal muscle from 8-10 days embryo only the embryo-specific isoform was detected. In extracts from 15 days embryo, it was noted that both the embryonic form and adult form co-existed. However, in 21 days embryo, the embryonic form disappeared, and the adult form was detected. These data suggested that the embryonic and the adult form of alpha-actinin are distinct gene products, and that during skeletal myogenesis in ovo, one class of alpha-actinin is replaced by a new class of alpha-actinin polypeptide,and the latter is maintained into adulthood. Filamin, gelsolin, vinculin and calpacin expression during skeletal myogenesis was also studied.
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