• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Immunochemical Studies on Phosphonolipids

Research Project

Project/Area Number 60580131
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 物質生物化学
Research InstitutionShiga University

Principal Investigator

SUGITA MUTSUMI  Faculty of Liberal Arts & Education, Shiga University, Professor, 教育学部, 教授 (20024937)

Project Period (FY) 1985 – 1986
Project Status Completed (Fiscal Year 1986)
Budget Amount *help
¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1986: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1985: ¥900,000 (Direct Cost: ¥900,000)
KeywordsPhosphonolipid(s) / Anti-phospholipid antibody / ホスホノ脂質抗体
Research Abstract

1. Specific antisera against ceramide 2-aminoethylphosphonate (CAEPn) from spermatozoa of the fresh-water bivalve, Hyriopsis schlegelii, were raised in rabbits.
2. The IgG fraction prepared from the antisera was found to agglutinate the liposome containing CAEPn, but it did not agglutinate those of containing other phosphono- and phospholipids.
3. The specificity was further examined by enzyme linked immunosorbent assay (ELISA technique), from which it was concluded that the antigenic determinants are the C-P bond, the free amino group and the ceramide portion (possibly the acid-amide bond).
4. The immunoquantitative analysis of CAEPn in the several fresh- and sea-water shellfish by ELISA technique indicated that these shellfish contained CAEPn amounting to 5 to 40% of the alkali-stable lipids.
5. The immune-reaction of liposomes containing an acidic glycolipid (uronic acid-containing glycolipid) with CAEPn or without CAEPn was studied. The immune-reactivity of the acidic glycolipid and the anti-glycolipid antibody depended on the CAEPn content in the liposome.

Report

(1 results)
  • 1986 Final Research Report Summary
  • Research Products

    (6 results)

All Other

All Publications (6 results)

  • [Publications] Sugita,M.;Nakae,H.;Yamamura,T.;Takamiya,Y.;Itasaka,O.;Hori,T.: J.Biochem.98. 27-34 (1985)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Itasaka,O.;Sugita,M.;Muraoka,S.;Tamari,M.;Hori,T.: Mem.of the Faculty of Ed.,Shiga Univ.35. 53-58 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] 杉田陸海,板坂修,堀太郎: 油化学.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Sugita, M.: "The Occurrence of Glycosphingolipids Containing Mannose in the Sea-Water Bivalve, Meretrix lusoria (Hamaguri)" J. Biochem.98. 27-34 (1985)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Itasaka, O.: "Biosynthesis of Ceramide 2-aminoethylphosphonate from Ceramide and Cytidine-monophosphate-2-aminoethylphosphonate in Bivalve Liver Homogenate" Mem. of the Faculty of Ed., Shiga Univ.35. 53-58 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Sugita, M.: "Immunochemical Studies on Phosphonolipids: Anti-Ceramide 2-aminoethylphosphonate antibodies" J. Jap., Oil Chemist's Soc.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary

URL: 

Published: 1987-03-31   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi