• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Structure and Function of Animal Lectin, and Their Role in Biological Regulation.

Research Project

Project/Area Number 60580139
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 物質生物化学
Research InstitutionTeikyo University

Principal Investigator

KASAI Ken-ichi  Faculty of Pharmaceutical Sciences, Teikyo University, 薬学部, 教授 (40001052)

Co-Investigator(Kenkyū-buntansha) HIRABAYASHI Jun  Faculty of Pharmaceutical Sciences, Teikyo University, 薬学部, 助手 (40156691)
ODA Yuko  Faculty of Pharmaceutical Sciences, Teikyo University, 薬学部, 助手 (30129986)
OHYAMA Yuji  Faculty of Pharmaceutical Sciences, Teikyo University, 薬学部, 助手 (90129982)
Project Period (FY) 1985 – 1986
Project Status Completed (Fiscal Year 1986)
Budget Amount *help
¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1986: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1985: ¥1,400,000 (Direct Cost: ¥1,400,000)
KeywordsLectin / <beta> -Galactoside / Chick Embryo / Human Placenta / Amino Acid Sequence / Lack of Signal Sequence / Histchemistry / 結合組織
Research Abstract

In order to elucidate biological significance of vertebrate <beta> -galactoside-binding lectins, those from chicken, mouse and human were investigated. As to chemical structure, complete amino acid sequence of one of two chick isolectins (14K type) was determined. It proved to be a unique protein because it was difficult to find out significantly homologous sequence in the present data base. Complete sequence of its cDNA was also determined and it was consistent with the amino acid sequence. It was suggested that initiator methionine is removed after biosynthesis and resultant N-terminal is acetylated. The result raised a new question about the mechanism of transportation of the lectin to outside of cells. Partial amino acid sequences were determined on chicken 16K and human placenta lectins. Significant homologies to some parts of chick 14K lectin were found. This suggested strong phylogenic relation between these lectins. In the course of investigation on the distribution of the human lectin, umbilical cord was found to contain relatively large amount of the lectin. Purified preparation showed that placenta and umbilical cord contain the identical lectin. Fluorescent antibody technique showed that the lectin was distributed mainly in connective tissue of umbilical cord. Both normal and tumor tisuues of liver and lung were found to contain large amount of the lectin. Monoclonal antibodies for chick 14K lectin were prepared. One of them seemed to be very useful because it can recognize conformational change of the lectin. As to biological activity of this family of the lectins, human placenta lectin was found to be able to stimulate production of some tumor cell killing factors by mouse and human macrophages.

Report

(1 results)
  • 1986 Final Research Report Summary
  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Yuji Ohyama: Biochem.Biophys.Res.Commun.134. 51-56 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Yuko Oda: J.Biochem.99. 1063-1070 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] T.Kajikawa: Life Science. 39. 1177-1181 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Jun Hirabayashi: J.Biochem.101. 775-787 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Jun Hirabayashi: J.Biochem.101. (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Bφg-Hansen,T.C.Ochoa,J.L.,eds. Mamoru kaneko: Lectin Walter de Gruyter Co.,Berlin,New York. 6. (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Yuji Ohyama: "Nucleotide Sequence of Chick 14K <beta> -Galactoside-Binding Lectin mRNA" Biochem. Biophys. Res. Commun.134. 51-56 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Yuko Oda: "Monoclonal Antibodies against a <beta> -Galactoside-Binding Lectin of Chick Embryo" J. Biochem.99. 1063-1070 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] T. Kajikawa: "Release of Cytotoxin by Macrophages on Treatment with Human Placenta Lectin" Life Science. 39. 1177-1181 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Jun Hirabayashi: "Complete Amino Acid Sequence of 14kDa <beta> -Galactoside-Binding Lectin of Chick Embryo" J. Biochem.101. 775-787 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Jun Hirabayashi: "Further Characterization and Structural Studies on Human Placenta Lectin" J. Biochem.101. (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Mamoru Kaneko: Lectin Vol. 6. <beta> -Galactoside-Binding Lectin of Human Umbilical Cord. Purification, Characterization and Distribution, (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary

URL: 

Published: 1987-03-31   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi