| Project/Area Number |
60580140
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
物質生物化学
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| Research Institution | Teikyo University |
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Principal Investigator |
NAKAGAWA Yasuhito Faculty of Pharmaceutical Sciences, Teikyo Univ., Lecturer, 薬学部, 講師 (00119603)
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| Project Period (FY) |
1985 – 1986
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| Project Status |
Completed (Fiscal Year 1986)
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| Budget Amount *help |
¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1986: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1985: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | Macrophage / Alkylacylglycerophosphocholine / Molecular species / アラキドン酸 |
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| Research Abstract |
The purpose of this project is to know the feature of the metabolism of the molecular species of phospholipids, especially arachidonoyl molecular species and alkylacyl GPC which is a precursor of platelet activating factor, using high performance liquid chromatography which is developed by a head investigator. The main results are as following;
1) Phospholipids in rabbit alveolar macrophages were composed of at least 130 different molecular species. One of the predominant species in the total phospholipids was the 16:0-20:4 species of alkylacyl GPC. In arachidonoyl species, a high proportion of 20:4 was associated with the 16:0-20:4 species of alkylacyl GPC (20% of the total arachidonoyl species).
2) The rate of incorporation of [ <^3H> ]20:4 into individual species was investigated. The incorporation of 20:4 into phospholipids was greatly influenced by the nature of their structures. The specific radioactivity of the 16:0-20:4 species of alkylacyl GPC, which was a predominant species, was quite lower than other molecular species.
3) Alveolar macrophages significantly released 20:4 from phospholipids by the stimulation of the opsonized zymosan. To determine the source of 20:4, the relative degradation of individual species of phospholipids was examined. The amounts of 20:4 liberated from the 16:0-20:4 species of alkylacyl GPC was greatest, representing almost 50% of the total 20:4 released from phospholipids.
These resuts indicate that the 16:0-20:4 species of alkylacyl GPC may play an important role as a major intracellular storage and also source of 20:4 in alveolar macrophages.
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