| Project/Area Number |
60580144
|
|---|
| Research Category |
Grant-in-Aid for General Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
代謝生物化学
|
|---|
| Research Institution | Hokkaido University |
|---|
Principal Investigator |
ISHIMOTO Makoto Faculty of Pharmaceutical Sciences, Hokkaido University, Prof., 薬学部, 教授 (80001030)
|
|---|
| Project Period (FY) |
1985 – 1986
|
| Project Status |
Completed (Fiscal Year 1986)
|
| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1986: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1985: ¥1,700,000 (Direct Cost: ¥1,700,000)
|
|---|
| Keywords | sulfate-reducing bacteria / Desulfovibrio / Sulfite reductase / cytochrome <C_3> / cloning |
|---|
| Research Abstract |
DNA from Desulfovibrio vulgaris Miyazaki F was partially digested by a restriction enzyme Sau 3A. The 5-10 kbp fragments obtained by CsCl gradient centrifugation were ligated with plasmid pBR322 freated with BamHl and phosphatase. E. coli HB101 was transfected with the ligates. The conditions were investigated. Ten to forty per cent of the obtained transfectant contained the DNA fragments. About one thousand strains thus obtained were tested by the immunoreaction with antisera against sulfite reductase or cytochrome <C_3> but strains clearly producing the antigens were not found. Further efforts should be paid for screening from much more transfectants and yield of transfection should be improved using HD1.
|
