| Project/Area Number |
60580153
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
代謝生物化学
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| Research Institution | 14401 Osaka University |
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Principal Investigator |
KATO Yukio Faculty of Dentistry , Instructor, Osaka University, 歯学部, 講師 (10112062)
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| Co-Investigator(Kenkyū-buntansha) |
HIRAKI Yuji Faculty of Dentistry , Research assistant, Osaka University, 歯学部, 助手 (40144498)
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| Project Period (FY) |
1985 – 1986
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| Project Status |
Completed (Fiscal Year 1986)
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| Budget Amount *help |
¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1986: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1985: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | Chondrocyte / Serum-free medium / Growth / Differentiation / プロテオグリカン |
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| Research Abstract |
We developed a new system for rabbit costal chondrocytes where cells proliferated rapidly and expressed the differentiated functions in serum-free medium. Addition of high density lipoprotein, transferrin, fibroblast growth factor, hydrocortisone and insulin to DMEM-Ham's F-12 medium (9:1) supported the growth of chondrocytes and their phenotypic expression when cells were seeded and maintained on extracellular matrix-coated dishes. Using these serum-free cultures, effects of transforming growth factor-beta (TGF-beta) and vanadate on chondrocyte growth and differentiation were examined. Our results indicate that TGF-beta stimulates the growth of chondrocytes and their synthesis of hyaluronic acid and chondroitin-sulfate proteoglycan. Vanadate, an inhibitor of phosphotyrosyl-protein phosphatase, also stimulated the growth of chondrocytes and chondroitin-sulfate proteoglycan synthesis at low concentrations (0.6-6 uM). At high concentrations (20-60 uM), vanadate suppressed chondroitin-sulfate proteoglycan synthesis and induced morphologic transformation. This system proved to be useful for studying the mechanisms involved in the control of chondrocyte growth and differentiation.
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