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Regulation of functions of RecA protein by the conformational change

Research Project

Project/Area Number 60580212
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 分子遺伝学・分子生理学
Research InstitutionOsaka University

Principal Investigator

OGAWA Tomoko  Faculty of Science, 理学部, 講師 (80028208)

Project Period (FY) 1985 – 1986
Project Status Completed (Fiscal Year 1986)
Budget Amount *help
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1986: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1985: ¥1,600,000 (Direct Cost: ¥1,600,000)
KeywordsThe activated form of RecA protein for the repressor cleavage / RecA protein / <phi> 80 repressor / The cleavage reaction of repressor by RecA protein / a role of the C-terminal region of RecA protein
Research Abstract

Purified RecA protein can cleave LexA protein and several prophage repressors ( <lambda> , P22 and 434 ) in vitro at a specific Ala-Gly peptide bonds in the middle of these polypeptide chains. This reaction requires single-stranded DNA, ATP (or ATP- <gamma> -S or dATP) and <Mg^(++)> . To learn what the activated form of RecA protein is and how the cleavage specificity is determined, we investigated the detail mechanisms on the cleavage reaction using <phi> 80, <lambda> and LexA repressors and RecA mutant proteins. Results showed that the RecA-mediated cleavage reaction of the repressors was controled with two processes. One is the formation of an active binary complex between RecA protein and single-stranded DNA. Another is the interaction of the active binary complex with repressor protein. The former process is likely to be controled by the C-terminal region of RecA protein, and the latter process seems to be stimulated by the conformational change of the repressor by interaction with a specific cofactor, such as dGpG or dApG.

Report

(1 results)
  • 1986 Final Research Report Summary
  • Research Products

    (15 results)

All Other

All Publications (15 results)

  • [Publications] H.Ogawa: Advance in Biophysics. 21. 135-148 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Y.Yamamoto: Journal of Biochemistry. 99. 1579-1590 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] T.Ogawa: Journal of Molecular Biology.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] T.Ogawa: Journal of Molecular Biology.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Y.Eguchi: Journal of Molecular Biology.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Y.Eguchi: Journal of Molecular Biology.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Y.Eguchi: Journal of Molecular Biology. in Press.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] 小川英行: 細胞工学. 4. 1197-1208 (1985)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] H. Ogawa: "General recombination: Functions and structure of RecA protein" Advances in Biophysics. 21. 135-148 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Y. Yamamoto: "Determination of the initiation sites of transcription and translation of the uvrD gene of Escherichia coli" Journal of Biochemistry. 99. 1579-1590 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] T. Ogawa: "Organization of the early region of bacteriophage <phi> 80: Genes and proteins" Journal of Molecular Biology.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Y. Eguchi: "Cleavage of <phi> 80CI repressor by RecA protein" Jpurnal Molecular Biology.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] T. Ogawa: "Transcriptional regulation of early functions of bacteriophage <phi> 80" Journal of Molecular Biology.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Y. Eguchi: "Two different forms of RecA protein in cleavage of repressor" Journal of Molecular Biology.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Y. Eguchi: Stimulation of RecA-mediated cleavage of <phi> 80 repressor by deoxydinucleotides Journal of Molecular Biology.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary

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Published: 1987-03-31   Modified: 2016-04-21  

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