Application of the ion selective field effect transistors (ISFET) on the measurement of fast reactions with biological importance
| Project/Area Number |
60580215
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
分子遺伝学・分子生理学
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| Research Institution | Jichi Medical School |
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Principal Investigator |
OHNO Koki Jichi Medical School, 医学部, 講師 (30049085)
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| Project Period (FY) |
1985 – 1986
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| Project Status |
Completed (Fiscal Year 1986)
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| Budget Amount *help |
¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1986: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1985: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | ISFET / Hydrogen ion / reaction kinetics / rapid mixing method / イオン輸送 |
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| Research Abstract |
1. The purpose of the project was the introduction of ISFET (ion selective field effect transistor) into the field of basic biological research involving the fast movement of the hydrogen ion ( <H^+> ). 2. A rapid mixing machine in which an ISFET was incorporated was constructed to check the usefulness of the semiconductor chip. 3. A model reaction involving pH change was started by mixing two solutions, HC <O_3> and HCl , and the resulting pH change was followed by a pH-indicator (BPB) and the ISFET. The temperature of the reaction was varied between 20 and 45゜C and the result from the optical method (pH-indicator) and the electrical method (ISFET) were compared. 4. The two methods gave almost the same reaction rate over the temperature rage measured. 5. In conclusion , ISFET is applicable for the measurement of fast pH change ( 10 ms ) with a sensitivity of about 0.005 pH unit.
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Report
(1 results)
Research Products
(6 results)
