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Development of Immunoanalyzer Based on Gate Function of Protein Membrane

Research Project

Project/Area Number 60850141
Research Category

Grant-in-Aid for Developmental Scientific Research

Allocation TypeSingle-year Grants
Research Field 工業分析化学
Research InstitutionTokyo University of Agriculture and Technology

Principal Investigator

MATSUOKA Hideaki  Tokyo University of Agriculture and Technology,Faculty of Technology,Associate Professor, 工学部, 助教授 (10143653)

Co-Investigator(Kenkyū-buntansha) ANDO Yutaka  Tokyo University of Agriculture and Technology,Reserch Assosiate, 工学部, 助手 (50107158)
NISHIDA Kenzo  Tokyo University of Agriculture and Technology,Faculty of Technology,Professor, 工学部, 教授 (30014989)
Project Period (FY) 1985 – 1986
Project Status Completed (Fiscal Year 1986)
Budget Amount *help
¥5,000,000 (Direct Cost: ¥5,000,000)
Fiscal Year 1986: ¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1985: ¥2,600,000 (Direct Cost: ¥2,600,000)
KeywordsGate functional membrane / Immunoassay / Flavour tracer / Protein membrane / Immunoanalyzer / Human olfactory function / ヒト嗅覚
Research Abstract

A novel system of immunoassay has been developed based on the gate function of protein membrane. A monolayer or similar thin layer of protein prepared on the surface of aqueous phase could inhibit the permeation of a flavour compound,versalide.A mixture of antigen (Candida albicans, diameter ca.4 <micro> m) and antibody (immunoglobulin G (IgG)) was reacted at 30゜C for 10 min, and then added on a phosphate buffer solution saturated with versalide. Unreacted IgG remained on the surface of the water. Finally the unreacted IgG covered the whole surface, as monitored by human olfactory function.The amount of added mixture necessary for the coverage was correlated with the concentration of antigen, C.albicans.Antibody bound latex beads were also prepared for the future application to immunoassay for proteins,peptides,and drugs. Immunoanalyzer system was composed of conical reaction cell unit, automatic level controller, and controlling system including a program for operating the whole system.

Report

(2 results)
  • 1986 Final Research Report Summary
  • 1985 Annual Research Report
  • Research Products

    (11 results)

All Other

All Publications (11 results)

  • [Publications] Hideaki MATSUOKA: Anal.Lett.(1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Eiichi TAMIYA: Biosensors. (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] 松岡英明: 日本化学会誌.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] 清山哲郎: "バイオセンサ実用便覧" フジテクノシステム, 780 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] 片岡照栄: "センサハンドブック" 培風館, 1177 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Hideaki MATSUOKA: "Pulse Immunoassay for Candida albicans" Anal. Chem.57. 1998-2002 (1985)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Hideaki MATSUOKA: "Application of Linear Image Sensor to Immunoassay Based on Turbidimetry of Antibody-bound Latex Beads" Anal. Lett.(1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Eiichi TAMIYA: "Pulse Immunoassay for Human Immunoglobulin G Using Antibody Bound Latex Beads" Biosensors. (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Hideaki MATSUOKA: "Bioreceptor Model with Use of Gate Functional Membrane (in Japanese)" J. Chem. Soc. Jpn.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Shoei KATAOKA: Baifukan Co.Sensor Handbook, 1177 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Analytical Chemistry. 57. 997 (1985)

    • Related Report
      1985 Annual Research Report

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Published: 1987-03-31   Modified: 2016-04-21  

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