| Project/Area Number |
60850152
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| Research Category |
Grant-in-Aid for Developmental Scientific Research
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| Allocation Type | Single-year Grants |
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| Research Field |
有機工業化学
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| Research Institution | Institute of Industrial Science, University of Tokyo |
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Principal Investigator |
SENO Manabu Institute of Industrial Science, University of Tokyo, 生産技術研究所, 教授 (40013099)
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| Co-Investigator(Kenkyū-buntansha) |
MIYATA Teruo Japan Biomedical Material Research Center, 所長
KUROYAGI Yoshimitsu School of Medicine, Kitasato University, 医学部, 講師 (80170140)
YOSHISATO Katsutoshi School of Medicine, Kitasato University, 医学部, 助教授 (20095516)
SHIOYA Nobuyuki School of Medicine, Kitasato University, 医学部, 教授 (80050376)
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| Project Period (FY) |
1985 – 1986
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| Project Status |
Completed (Fiscal Year 1986)
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| Budget Amount *help |
¥7,000,000 (Direct Cost: ¥7,000,000)
Fiscal Year 1986: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1985: ¥5,600,000 (Direct Cost: ¥5,600,000)
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| Keywords | Artificial skin / Wound dressing / Poly(amino acid) / Poly-L-leucine / Burn treatment / 生体適合性 / 生体適合性材料 / 医用材料 / フィブリン / 組織適合性 |
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| Research Abstract |
The safe coverage of skin defect and suppresion of bacterial growth are very important for the treatment of burn injury. In the present work our attention is focussed on the development of new temporary wound dressing capable of releasing antibacterial drug. The medicated wound dressing was prepared from poly-L-leucine and silver sulfadiazine. The evaluation of medicated wound dressing was carried out in vitro and in vivo. This medicated wound dressing has a unique structure composed of two layers, thin outer membrane and supporting sponge layer. It was proved from animal tests with rats that this medicated wound dressing is able to protect full-thickness skin defects for a long period and to produce a good tissue bed for autograft.
The suppression of bacterial growth was studied on agar surfaces seeded with Pseudomonas aeruginosa. When the medicated wound dressing was placed on the agar seeded with <10^7> P.a./ <cm^2> , no bacteria was observed beneath the dressing after one day. Furthermore, the medicated wound dressing was evaluated on the full-thickness skin defect of mouse seeded with <10^7> P.a. After the dressing was applied to mouse with freshly contaminated pannicular wound for one day, the panniculus muscles were excised for homogenization and quantitation of the residual bacterial content. In control tests, various wound dressings were examined and only in the present wound dressing no bacteria was pbserved from the pannicular wound. It was proved that this medicated wound dressing is able to protect the full-thickness skin defect and to suppress the bacterial growth effectively.
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