Development of new ultra micro analytical system based on the acridine fluorometry
| Project/Area Number |
60860012
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| Research Category |
Grant-in-Aid for Developmental Scientific Research
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| Allocation Type | Single-year Grants |
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| Research Field |
製造化学・食品
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| Research Institution | TOHOKU UNIVERSITY |
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Principal Investigator |
MEGURO Hiroshi Faculty of Agriculture, Tohoku University, 農学部, 教授 (30005590)
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| Co-Investigator(Kenkyū-buntansha) |
NISHIDA Yoshihiro Faculty of Agriculture, Tohoku University, 農学部, 助手 (80183896)
OHRUI Hiroshi Faculty of Agticulture, Tohoku University, 農学部, 助教授 (20100050)
鈴木 建夫 東北大学, 農学部, 助手 (80005652)
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| Project Period (FY) |
1985 – 1987
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| Project Status |
Completed (Fiscal Year 1987)
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| Budget Amount *help |
¥7,500,000 (Direct Cost: ¥7,500,000)
Fiscal Year 1987: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1986: ¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1985: ¥3,700,000 (Direct Cost: ¥3,700,000)
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| Keywords | N-(9-Acridinyl)maleinide / thiol / 9-Bromomethylacridine / fruorometry / Carboxylic acid / fat / 脂肪 / 高速液体クロマトグラフィー / セレノール / N-(9-アクリニジル)マレイミド / セレノシステイン / ゼレノシスチン / メチオニン-γ-リアーゼ / リポ酸 / 螢光分析 |
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| Research Abstract |
Two fluorometry based on 1:N(9-Acridinyl)maleimide (NAM) for SH,SeH and SO_2 and 2:9-Bromomethylacridine (9-BrMA) for RCOOH were developed.
(1) A new synthetic method for NAM was developed. The yield was greatly improved.
(2) A new fluorometric reaction of NAM and SeH was developed. Electrical reaction of -Se-Se- to SeH was combined new dluorometry of seleno cysteins was designed.
(3) NAM fluotometry was used in enzymatic analysis of methionine after convertion to homocysteind.
(4) A new fruorometric reagent 9-BrMa was used to label carboxylic acid on high performance liquid chromatography. The hydrolysis was also designed to determine the carboxylic acid composition of fat.
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Report
(3 results)
Research Products
(9 results)
