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DEVELOPMENT OF A ULTRAHIGH-VACUUM CHAMBERF TGO FACILITATE DEEP-ETCHING PREPARATION FOR BIOLOGICAL ELECTRON MICROSCOPY.

Research Project

Project/Area Number 60870001
Research Category

Grant-in-Aid for Developmental Scientific Research

Allocation TypeSingle-year Grants
Research Field General anatomy (including Histology/Embryology)
Research InstitutionYAMAGATA UNIVERSITY

Principal Investigator

TONOSAKI Akira  YAMAGATA UNIVERSITY SCHOOL OF MEDICINE, 医学部, 教授 (90004572)

Co-Investigator(Kenkyū-buntansha) 会田 瑳武朗  日本電子株式会社, 電子工学機器技術本部, 研究室長
WATANABE Hiroshi  YAMAGATA UNIVERSITY SCHOOL OF MEDICINE, 医学部, 助手 (20091845)
渡辺 晧 (渡辺 皓)  山形大学, 医学部, 助教授 (80004662)
TOKUNAGA Fumio  TOHOKU UNIVERSITY FACULTY OF SCIENCE, 理学部, 助教授 (80025452)
AITA Saburo  JAPAN ELECTRON OPTICS LABORATORY, CO. LTD.
松尾 達也  日本電子(株), 部長
Project Period (FY) 1985 – 1987
Project Status Completed (Fiscal Year 1987)
Budget Amount *help
¥13,000,000 (Direct Cost: ¥13,000,000)
Fiscal Year 1987: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1986: ¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1985: ¥8,500,000 (Direct Cost: ¥8,500,000)
KeywordsClean vacuum / Deep-etching / Cryopump / Vacuum evaporation / 電顕試料 / 凍結レプリカ法 / 真空蒸着装置 / 電顕試料作製法 / フリーズ・エッチング法
Research Abstract

SUB-CHAMBER HOUSING A DEEP-ETCHING STAGE AND PROVEDED WITH A CRYOPUMP, CRYO-U6 (ALVAC), WAS CONJUGATED WITH A UNIT OF VACUUM EVAPORATER. JFD-7000 (JEOL), WHICH HAD BEEN EARLIER DEVELOPED BY THE MEMBERS OF THE PRESENT PROJECT AND WAS CHARACTERIZED BY ITS TWO-WAY EVACUATION BY MEANS OF TWO SETS OF OIL-DIFFUSION PUMPS. MANY VACUUM-SPECIALISTS WERE SCEPTICAL OF SUCH A PLAN BECAUSE OF APPARENT DEMERIT OF HYBRIDIZING OF so-called clean and wet vacuum system. a vacuum of 10^<-9> ORDER WAS EVENTUALLY OBTAINED IN NUDE-GAUGE MONITORING MODE IN THE SUB-CHAMBER.
THE SUB- AND MAIN CHAMBERS WERE ACTUALLY CONNECTED ONLY UPON USE BY A PASSAGE TGHROUGH WHICH A SPECIAMEN-HOLDER WAS PUSHED IN AND PULLED OUT. THE PASSAGE OTHERWISE STAYED CLOSED BY A SLIDE-VALVE TO KEEP THE VACUUM SYSTEM SEPARATED FROM EACH OTHER. BAKING HEATER AND METAL SEALS, BOTH REQUIREMENT FOR CLEAN-VACUUM MAINTAINANCE, WERE GIVEN UP BECAUSE THEY WERE SUPPOSED TO TAKE TOO MUCH SPACE OF DESIGNED SUB-CHAMBERS AND LOOKED UNREALISTIC FOR SUCH A CASUAL WORK AS FREEZE-ETCHING EXPERIMENTS, USED TO BE REPEATED INCESSANTLY EVERY TIME TO RUIN THE VACUUM.

Report

(3 results)
  • 1987 Final Research Report Summary
  • 1986 Annual Research Report
  • 1985 Annual Research Report
  • Research Products

    (26 results)

All Other

All Publications (26 results)

  • [Publications] H.Watanabe et al.: Anat.Rec.214. 82-88 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] H.Watanabe et al.: cell tiss.Res.245. 413-421 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] A.Tonosaki et al. T.Imura,S.Maruse,T.Suzuki,eds.: In:Proc. XIth Int. Cong. Electron Microsc., Kyoto. III. 1848 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] H.Watanabe et al. T.Imura,S.Maruse,T.Suzuki,eds.: In:Proc. XIth Int. Cong. Electorn Microsc., Kyoto. III. 2487 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] M.Ishikawa et al.: Cell Tiss.Res.249. 241-246 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] A.Tonosaki et al.: Neurosci.Ress. Suppl.6. S107-S118 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] M.Ishikawa et al.: Cell Tiss.Res.(1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] T.Kikuchi et al.: Acta Otolaryngol.(1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] WATANABE H, WASHIOKA H, TONOSAKI A: ""CHOLINERGIC" POSTSYNAPTIC MEMBRANES OF BULLFROG SYMPATHETIC "CHOLINERGIC" POSTSYNAPTIC MEMBRANES OF BULLFROG SYMPATHETIC "CHOLINERGIC" POSTSYNAPTIC MEMBRANES OF BULLFROG SYMPATHETIC GANGLIA: ELECTRON MICROSCOPY OF THIN SECTIONS AND FREEZE-FRACTURE REPLICAS." ANAT REC. 214. 82-88 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] WATANABE H, TONOSAKI A: ""SIF" CELLS IN THE SYMPATHETIC GANGLIA OF THE BULLFROG, RANA CATESBEIANA: VARIETY IN POPULATION AND INNERVATION." CELL TISS RES. 245. 413-421 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] TONOSAKI A, WASHIOKA H, TOKUNAGA F, KATAOKA M, WATANABE H: "STRUCTURE OF THE RED MEMBRANE AND ASSOCIATRED FILAMENTS IN HALOBACTERIUM HALOBIUM." PROC XITH INT CONG ELECTRON MICROSC, KYOTO (T IMURA, S MARUSE, T SUZUKI, EDS). III. 1845-1848 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] WATANABE H, WASHIOKA H, TONOSAKI A: "GAP JUNCTGIONS ASSOCIATED WITH MEMBRANCE SKELETONS." PROC XITH INT CONG ELECTRON MICROSC, KYOTO (T IMURA, S MARUSE, T SUZUKI, EDS). III. 2487- (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] ISHIKAWA M, TAKAO M, WASHIOKA ', TOKUNAGA F, WATANABE H, TONOSAKI A: "DEMONSTRATION OF ROD AND CONE PHOTORECEPTORS IN THE LAMPREY RETINA BY FREEZE-REPLICATION AND IMMUNOFLUORESCENCE." CELL TISS RES. 249. 241-246 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] TONOSAKI A, WASHIOKA H, HARA M, ISHIKAWA M, WATANABE H: "GAP JUNCTIONS AND SYNAPTIC RELATIONS OF HORIZONTAL CELLS IN LAMPREY RETINA." NEUROSCI RES (SUPPL). 6. S107-S118 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] ISHIKAWA M, WATANABE H, HISATOMI O, KOIKE Y, TONOSAKI A: "DEMONSTARATION OF ROD AND CONE PHOTORECEPTORS IN THE LAMPREY RETINA BY LECTIN CYTOCHEMISTRY." CELL TGISS RES IN PRESS. (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] KIKUCHI TG, TONOSAKI A, TAKASAKA T: "DEVELOPMENT OF APICAL-SURFACE STRUCTURES OF MOUSE OTIC PLACODE." ACTA OTOLARYNGOL IN PRESS. (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Tonosaki A. et al.: Proc.【XI】Int.Cong.Electr.Microsc.3. 1845-1848 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] Watanabe H.et al.: Anat.Rec.214. 82-88 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] Watanabe H.et al.: Cell Tiss.Res.245. 413-421 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] Watanabe H.et al: Proc.【XI】 Int.Cong.Electr.Microsc. 3. 2487-2488 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] Ishikawa M.et al.: Cell Tiss.Res.(1987)

    • Related Report
      1986 Annual Research Report
  • [Publications] Tonosaki A.et al.: Neurosci.Res.(1987)

    • Related Report
      1986 Annual Research Report
  • [Publications] 外崎昭: "新生理科学大系【I】.興奮膜の生理学" 医学書院, 336 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] Journal of Electron Microscopy Technique. 2:. 187-192 (1985)

    • Related Report
      1985 Annual Research Report
  • [Publications] Cell and Tissue Research. 241:. 523-528 (1985)

    • Related Report
      1985 Annual Research Report
  • [Publications] Anatomical Record. 214:. 82-88 (1986)

    • Related Report
      1985 Annual Research Report

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Published: 1987-03-31   Modified: 2016-04-21  

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