| Project/Area Number |
60870007
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| Research Category |
Grant-in-Aid for Developmental Scientific Research
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| Allocation Type | Single-year Grants |
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| Research Field |
環境生理学(含体力医学･栄養生理学)
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
MORIMOTO Taketoshi Kyoto Prefectural University of Medicine, 医学部, 教授 (30079694)
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| Co-Investigator(Kenkyū-buntansha) |
青海 隆 堀場製作所, 開発本部, 部長
ITOH Toshiyuki Kyoto Prefectural University of Medicine, 医学部, 助手 (90168360)
NOSE Hiroshi Kyoto Prefectural University of Medicine, 医学部, 助手 (40128715)
AOMI Takashi Horiba Ltd.
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| Project Period (FY) |
1985 – 1986
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| Project Status |
Completed (Fiscal Year 1986)
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| Budget Amount *help |
¥4,200,000 (Direct Cost: ¥4,200,000)
Fiscal Year 1986: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1985: ¥2,800,000 (Direct Cost: ¥2,800,000)
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| Keywords | Continuous determination / Plasma Na / Plasma K / Circulating blood / ガラス電極 / Naガラス電極 / 意識下ラット |
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| Research Abstract |
A new continuous measuring system for blood Na and K concentrations was developed. A small extracorporeal circulation was made and the blood was passed through Na and K sensitive glass electrodes. Applying the system to conscious rats and dogs, applicability of the method for physiological analyses was shown.
Measurements of [Na] and [K] were made using Na and K sensitive glass electrodes and a reference electrode mounted on a temperature control unit of an electrolyte analyzer (SERA-230, Horiba, Kyoto). In the present system, the liquid junction used in the commercial analyzer was replaced by 3.3 mol/1 KCl agar bridge adjusting pH and Na concentration of the agar to those of blood. The accuracy of the determination was about 1.5 meq/1 of Na and less than 0.1 meq/1 of K concentration within physiological range. The effect of erythrocytes with 42 to 51% of hematocrit on reading was within 0.1 mV.
Animal experiments were performed on rats and dogs, and used for the analysis of drinking behavior. The results showed that the system is sensitive enough to detect the changes in Na concentration consequent to drinking behavior and also to detect difference of salinity of drinking water. These results indicate that the present method is a potential tool for not only for physiological studies but also for clinical use in the fields of nephrology and cardio-vascular surgery.
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