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Expoloration of spasmogenic factors and its antagonist for cerebrovasculor spasm

Research Project

Project/Area Number 60870010
Research Category

Grant-in-Aid for Developmental Scientific Research

Allocation TypeSingle-year Grants
Research Field General pharmacology
Research InstitutionKyoto University

Principal Investigator

FUJIWARA Motohatsu  Department of Pharmacology, Faculty of Medicine, 医学部, 教授 (90025536)

Co-Investigator(Kenkyū-buntansha) OKAMOTO Shinichiro  Department of Neurosurgery, Faculty of Medicine, 医学部, 助手 (40160717)
MIWA Soichi  Same to above, 医学部, 助手 (40157706)
TANIGUCHI Takashi  Same to above, 医学部, 講師 (10111957)
NARUMIYA Shu  Department of Pharmacology, Faculty of Medicine, 医学部, 助教授 (70144350)
KURAHASHI Kazuyoshi  Radioisotope Research Center, 放射性同位元素総合センター, 助教授 (10025653)
Project Period (FY) 1985 – 1987
Project Status Completed (Fiscal Year 1987)
Budget Amount *help
¥6,500,000 (Direct Cost: ¥6,500,000)
Fiscal Year 1987: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1986: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1985: ¥3,500,000 (Direct Cost: ¥3,500,000)
KeywordsSubarachnoid hemorrhage / Cerebrospinal fluid / Endothelium-dependent contraction / 脳血管攣縮 / クモ膜下出血 / 内皮細胞依存性収縮因子 / TX【A_2】様物質 / 脳脊髄液 / 脳血管 / 内皮細胞
Research Abstract

We have reported the ACh produces endothelium-dependent contractile (EDC) response in canine cerebral artery, and a possible endothelium-derived contracting factor (EDCF) is TXA_2. The present experiments were undertaken to elucidate whether the cerebrospinal fluid (CSF) collected from the patients suffered from subarachinoid hemorrhage (SAH) causes EDC response in canine cerebral artery, and to determine spsmogenic substances in the fulid and explore its antagonist. The sample prepared from the CSF collected from the patients suffered from SAH caused EDC response in canine cerebral artery and the response was inhibited by phospholipase inhibitor, lipooxygenase inhibitor, cyclooxygenase inhibitor, TXA_2 synthetase inhibitor and TXA_2 antagonist. ATP,ADP and AMP, whith are released from hemolysate and platelet,caused EDC response in canine cerebral artery. The EDC response induced by A-23187 was more strongly inhibited by nifedipine than that indeced by TXA_2 analogue, STA_2. The present studies demonstrated that CSF obtained from the patients suffered from SAH stimulates endothelium and releases TXA_2-like substances, requiring Ca_<2+> mobilization. It was quggested that possible substances to stimulate endothelium are adenine nucleotides and the spasm following SAH may be inhibited by TXA_2 synthetase inhibitor and its antagonist, or specific Ca_<2+> antagonists against TXA_2-induced Ca^<2+> mobilization.

Report

(3 results)
  • 1987 Final Research Report Summary
  • 1986 Annual Research Report
  • 1985 Annual Research Report
  • Research Products

    (31 results)

All Other

All Publications (31 results)

  • [Publications] Hachiro Usui: Japan J.Pharmacol.228-231 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Hiroaki Shirahase: Blood Vessels. 24. 117-119 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Yoshinobu Akimoto: Japan J.Pharmacol.44. 506-509 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Kazutoshi Fujii: Br.J.Pharmacol.90. 355-363 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Hachiro Usui: Jpn. J. Clin. Pharmacol. Ther.18. 147-148 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Hiroaki Shirahase: J. Cardeovasc. Pharmacol.10. 517-522 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Hiroaki Shirahase: Life Sciences. 42. 437-455 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Hiroaki Shirahase: (Submitted to J. Pharmacol. Exp. Ther.).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Hiroaki Shirahase: (Submitted to J. Pharmacol. Exp. Ther.).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Hiroaki Shirahase: (Submitted to J. Pharnacol. exp. Ther.).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Hachiro Usui: "Neuronal Messengers in Vascular Function" A. Nobin, C.Owman and B. Arneklo-nobin, 10 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Hachiro Usui: "Endothelium-dependent vasontraction in response to noradenaline in the canine cerebral artery" Japan J. Pharmacol.44. 228-231 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Hiroaki Shirahase: "A possible rore of theomboxane A2 in endothelium in maintaining resting tone and producing contractile response to acetylcholine and arachidonic acid in canine cerebral arteries" Blood Vessels. 24. 117-119 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Yoshinobu Akimoto: "Nitroglycerin-sensitive and-resistant contractions mediated by receptor-operated calcium channels in rabbit ear artery" Japan J. Pharmacol.44. (1987 506-509)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Kazutoshi Fujii: "Regional differences of reactivity to stimulants in the dog portal tree" Br. J. Pharmacol.90. 355-363 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Hachiro Usui: "Contractile response due to endothelium derived contracting factors in dog cerebral artery by cerebrospinal fulud obtained from patients with subarachinoid hemorrhage" Jpn. J. Clin. Pharmacol. Ther.18. 147-148 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Hiroaki Shirahase: "Possible rore of endothelial thromboxane A_2 in the resting tone and contractile responses to acetylcholine and arachidonic acid in canine cetebral arteries" J. Cargiovasc. Pharmacol.10. 517-522 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Hiroaki Shirahase: "Endothelium-dpendent contraction indeced by nicotine in isolated canine basilat artery-possible involvement of a thromboxane A_2 (TXA_2) like substance" Lide Sciences. 42. 437-455 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Hiroaki Shirahase: "Different effects of prostaglandin I2 on the canine cerebral artery (EDCF-type) and on the canine coronary and mesenteric arteries (EDRF-type)"

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Hiroaki Shirahase: "Canine cerebrovasular tone maintained by putative EDCF (TXA_2) - nifedipine-sensitive and resistant Ca^<2+> mobilization"

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Hiroaki Shirahase: "An endothelium-dependent contraction induced by A-23187, Ca^<2+> ionophore in canine basilar artery"

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Hiroaki Shirahase: "Endothelium-dependent contraction and independent relaxation induced by adenine nucleotides and nucleoside in canine basilar artery"

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Hachiro Usui: A. Nobin, C. Owman and B. Rneklo-Nobin. Neuronal Messengers in Vascular Function, 10 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] H.Usui: Japan.J.Pharmacol.40(Supp.). -172P (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] H.Shirahase: Japan.J.Pharmacol.40(Supp.). -263P (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] H.Shirahase: J.Cardiovasc.Pharmacol.(1987)

    • Related Report
      1986 Annual Research Report
  • [Publications] H.Shirahase: Blood Vessles. (1987)

    • Related Report
      1986 Annual Research Report
  • [Publications] T.Tsukahara: Stroke.

    • Related Report
      1986 Annual Research Report
  • [Publications] H.Usui: "Possible involvement of prostanoids in cholinergic contractile response of canine cerebral artery.In"Neural Regulation of Brain Circulation"." C.Owman and J.E.Hardebo.Elsevier Science Publishers., P261-272 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] H.Usui: "Endothelium-dependent vasoactivation of canine cerebral artery by cerebrospinal fluid obtained from the patients suffered from subarachnoid hemorrhage.In"Neuronal Messengers in Vascular Function."" A.Nobin,C.Owman and B.Arneklo-Nobin.Elsevier Science Publishers., (1987)

    • Related Report
      1986 Annual Research Report
  • [Publications] J.Cardiovascular Pharmacology. 7-Suppl.3. (1985)

    • Related Report
      1985 Annual Research Report

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Published: 1987-03-31   Modified: 2016-04-21  

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