• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

An equipment for microdetermination of superoxide production of nentrophils.

Research Project

Project/Area Number 60870034
Research Category

Grant-in-Aid for Developmental Scientific Research

Allocation TypeSingle-year Grants
Research Field Pediatrics
Research InstitutionKobe University

Principal Investigator

NAKAMURA Hajime  Kobe University School of Medicine Department of Pediatrics, Lecturer, 医学部付属病院, 講師 (40030978)

Co-Investigator(Kenkyū-buntansha) 生越 義昌  株式会社アローズ, 取締役社長
OGOSHI Yoshiaki  Arrows Co. President
Project Period (FY) 1985 – 1986
Project Status Completed (Fiscal Year 1986)
Budget Amount *help
¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1986: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1985: ¥1,000,000 (Direct Cost: ¥1,000,000)
KeywordsSuperoxide / Neutrophils / PMA / スーパーオキサイド産生能 / 好中球機能 / 微量測定法
Research Abstract

We have developped anequipment for mictrodetermination of superoxide production of polymorphonuclear neutrophils (PMNs).
This equipment consists of special double beam spectrophotometer (550nm - 540nm) and data analysis system by microcomputer for eliminating a noise due to cells in the samples.
PMNs were stimulated by either phorbol myristate acetate or Concanavalin A plus Cytocharasin D, Or FMLP, and their optimal Quantities were 0.01 to 5 ug for PMNs riched plasma, 0.1 to 5 ug for separated PMNs by PMA; 10 to 100 ug for Con A plus Cyt D, 0.1 to 1 umoles for FMLP. Only 5 to 10x10^4 PMNs were required for single/assay.
By PMA, the superoxide production rates showed a good correlation between the value of plasma riched PMNs and the value of separated PMNs. By Con A plus Cyt D, The superoxide production rates showed a good correlation between the value of plasma riched PMNs and the values of separated PMNs in Newborns, however in adult, the values obtained from plasma riched PMNs were lower than those obtained from separated PMNs.
This new device provided us to determine superoxide production rates of PMNs conveniently, and it is possible to determine it using plasma riched PMNs in newborn infants.

Report

(3 results)
  • 1986 Annual Research Report   Final Research Report Summary
  • 1985 Annual Research Report
  • Research Products

    (7 results)

All Other

All Publications (7 results)

  • [Publications] 中村 肇 他: 医学のあゆみ. 140. 987-988 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Hajime NAKAMURA: Biology of the Neonate. 52. 273-278 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Hajime,NAKAMURA: "AN IMPROVED EQUIPMENT FOR MAASURING SUPEROXIDE PRODUCTION OF NEUTROPHILS." IGAKU_NO AYUMI. 140. 987-988 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Hiajime,NAKAMURA: "INHIBITORY ACTION OF BILIRUBIN ON SUPEROXIDE PRODUCTION BY POLYMORPHONUCLEAR LEUCOCYTES." BIOLOGY OF THE NEONATE. 52. 273-278 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] 中村肇 他: 医学のあゆみ.

    • Related Report
      1986 Annual Research Report
  • [Publications] Hajime NAKAMURA: Biological Neonate.

    • Related Report
      1986 Annual Research Report
  • [Publications] Pediatric Research. 19-4. (1985)

    • Related Report
      1985 Annual Research Report

URL: 

Published: 1987-03-31   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi