| Project/Area Number |
60870043
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| Research Category |
Grant-in-Aid for Developmental Scientific Research
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| Allocation Type | Single-year Grants |
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| Research Field |
General surgery
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| Research Institution | Asahikawa Medical College |
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Principal Investigator |
MITO Michio Asahikawa Medical College, 医学部, 教授 (60000981)
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| Co-Investigator(Kenkyū-buntansha) |
MUNAKATA Takashi Asahikawa Medical College, 医学部, 助手 (10166233)
KASAI Shinichi Asahikawa Medical College, 医学部, 講師 (40091566)
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| Project Period (FY) |
1985 – 1986
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| Project Status |
Completed (Fiscal Year 1986)
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| Budget Amount *help |
¥10,800,000 (Direct Cost: ¥10,800,000)
Fiscal Year 1986: ¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 1985: ¥7,300,000 (Direct Cost: ¥7,300,000)
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| Keywords | hybrid artificial liver / isolated hepatocytes / fulminant hepatic failure / artificial liver support / hollow fiber membrane / ガラクトサミン肝不全 / Hybrid Atificial liver / 肝細胞保存 / 解毒機能 / 血液浄化法 |
|---|
| Research Abstract |
Fulminant hepatic failure (FHF) due to acute massive liver cell necrosis is a complex pathophysiological entity, and treatment is still unsatisfactory. Recently, artificial liver support (ALS) such as hemodialysis, hemoperfusion, and plasmapheresis have been used clinically to treat FHF. However, survival rate has not improved as expected although the number of patients regaining consciousness has increased. One reason for a low survival rate appears to be the lack of an effective therapeutic means of supporting the metabolic function of the damaged liver. We tried to develop a metabolic support device which used large quantities of isolated hepatocytes. The isolated hepatocytes were prepared from adult dog by the modified method of Berry & Friend. An experimental metabolic support device was developed for an animal model. About 40g of hepatocytes was used for the metabolic reactor. Hollow fiber separate membranes were placed in the hepatocytes chamber. In in-vitro perfusion tests, viability of hepatocytes and ATP concentration of the cell were well maintained over 10 hours. In in-vivo experiments using drug induced hepatic failure dog, the system worked safety and the survival time of the animal was prolonged significantly.
These results suggest the usefulness of artificial liver support system using isolated hepatocytes.
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