Establishment of Quality Evaluation Method for Crude Drugs by GC-MS

Research Project

Project/Area Number	60870077
Research Category	Grant-in-Aid for Developmental Scientific Research
Allocation Type	Single-year Grants
Research Field	Chemical pharmacy
Research Institution	Osaka University
Principal Investigator	YOSHIKAWA Masayuki Associate Professor, Faculty of Pharmaceutical Sciences, Osaka University, 薬学部, 助教授 (90116129)
Co-Investigator(Kenkyū-buntansha)	SHIBUYA Hirotaka Research Associate, Faculty of Pharmaceutical Sciences, Osaka University, 薬学部, 助手 (50116042)
Project Period (FY)	1985 – 1986
Project Status	Completed (Fiscal Year 1986)
Budget Amount *help	¥6,000,000 (Direct Cost: ¥6,000,000) Fiscal Year 1986: ¥2,000,000 (Direct Cost: ¥2,000,000) Fiscal Year 1985: ¥4,000,000 (Direct Cost: ¥4,000,000)
Keywords	GC-MS / Crude Drug / Quality Evaluation / Processing / Ginseng; Capillaris / Zedoary / 莪じゅつ / 生姜 / 菌ちん蒿
Research Abstract	It is the purpose of this research project to establish a quality evaluation method for crude drugs (e.g. ginseng, capillaris, zedoary, ginger), which are important ingredients of Chinese traditional herb formulae, by means of gas liquid chromatography-mass spectrometry. 1) Ginseng: The difference of lipophilic constituents between "white ginseng" and "red ginseng", which were prepared by processing of the same Panax ginseng root, has been clarified by GC-MS analysis. 2) Capillaris: The method for qualitative and quantitative analysis of choleretic principles in capillaris has been established by GC-MS. 3) Zedoary: The essential oil constituents in zedoaries originated in China, Taiwan, and Yakushima (Japan) were analized by GC-MS. It has been found that each essential oil gives a characteristic reconstructed ion current (RIC) chromatogram. 4) Ginger: Many useful findings have been obtained in comparison of GC-MS data for bitter principles of gingers originated in Kohchi Pref., Shizuoka Pref., China, and Vietnam.