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New Mechod on Evaluating Fibrinolytic Activity using Calormitry

Research Project

Project/Area Number 60870079
Research Category

Grant-in-Aid for Developmental Scientific Research

Allocation TypeSingle-year Grants
Research Field Physical pharmacy
Research InstitutionToyama Medical & Pharmaceutical Univerisity

Principal Investigator

UENO Masaharu  Department of Hospital Pharmacy, Toyama Medical & Pharmaceutical University, Associate Professor, 附属病院, 助教授 (40080197)

Co-Investigator(Kenkyū-buntansha) TAKAHASHI Kaoru  Department of Central Clinical Laboratory, Toyama Medical & Pharmaceutical Unive, 附属病院, 助教授 (70135028)
ADACHI Isao  Department of Hospital Pharmacy, Toyama Medical & Pharmaceutical University, Res, 附属病院, 助手 (30151070)
Project Period (FY) 1985 – 1987
Project Status Completed (Fiscal Year 1987)
Budget Amount *help
¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 1987: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1986: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1985: ¥1,900,000 (Direct Cost: ¥1,900,000)
KeywordsCalorimetry / Fibrinolytic activity / Microcalorimeter / Flow-throgh-Cell / Urokinase / Fibrinogen / Plasminogen / プラスミン / フィブリン / 活性定量 / 熱 / 活性 / 微少熱量計
Research Abstract

A novel method using calormetry on the evaluation of activity of fibrinolytic enzyme was developed. LKB microcaolrimeter equipped by flow-throug-cell was used. The protocol of the method was as follows: An enzyme and an excess amount of the substrate were mixed. The solution, in which the fibrinolytic reaction was in the steady state, was led to a thermodetector. The heat produced in unit time was measured. First, the reaction heat in two component system was measuted. Plasmin was used as a fibrinolytic enzyme. The reaction heat for fibrinogen, fibrin and casein as substrates of plasmin were measured. As fibrinogen showed the highest reaction heat, fibrinogen was adoptted as the substrate. Next, 3 component system i.e. urokinase, plasminogen and fibrinogen, was studied. Because of the practical 0 heat of the reaction from plasmininogen to plasmin, the heat observed in this system can be regraded as that of the reaction from fibrinogen to FDP by Plasmin. A linear relationship between UK concentration and observed heat was obtaind within the UK concentration of 0.5-20 unit/ml. Thus the method on evaluation of fibrinolitic activity using calorimetry was established. The methods using vescoelasticity and light transmittance were also studied.

Report

(3 results)
  • 1987 Final Research Report Summary
  • 1986 Annual Research Report
  • 1985 Annual Research Report
  • Research Products

    (7 results)

All Other

All Publications (7 results)

  • [Publications] 中川輝昭,安田晶子,鳥居塚和生,足立伊左雄,上野雅晴,堀越勇: 病院薬学. 12. 369-373 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] M.Ueno,M.Kimura,T.Nakagawa,Yasui,and I.Horikoshi.: Drug Interations. 11. 25-32 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] T. Nakagawa, A. Yasute, K. Toriizuka, I. Adachi, M. Ueno, and I. Horikoshi: "Measutement of Urokinase Activity Using Thrombelastgraph and Stability of Urokinase Preparation in Solution" Hospital Pharmacy. 12. 369-373 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] M. Ueno, M. Kimura, T. Nakagawa, T. Yasui, and I. Horikoshi: "Effects of Added Substance in Injections on Blood Coagulation, Fibrinolysis and Platelet Aggregation" Drug Interactions. 11. 25-32 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] M. Ueno, T. Nakagawa, N. Kawabata, and I. Hotikoshi: "Thermal Property of Fibrinolytic Reaction"

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] M. Ueno, T. Nakagawa, Mn. Saito, and I. Horikoshi: "%development of the Mechod of Fibrinolytic Activity"

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 中川輝昭,安田晶子,鳥居塚和生,足立伊左雄,上野雅晴,堀越勇: 病院薬学. 12. 369-372 (1986)

    • Related Report
      1986 Annual Research Report

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Published: 1987-03-31   Modified: 2016-04-21  

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