| Project/Area Number |
60870079
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| Research Category |
Grant-in-Aid for Developmental Scientific Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Physical pharmacy
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| Research Institution | Toyama Medical & Pharmaceutical Univerisity |
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Principal Investigator |
UENO Masaharu Department of Hospital Pharmacy, Toyama Medical & Pharmaceutical University, Associate Professor, 附属病院, 助教授 (40080197)
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| Co-Investigator(Kenkyū-buntansha) |
TAKAHASHI Kaoru Department of Central Clinical Laboratory, Toyama Medical & Pharmaceutical Unive, 附属病院, 助教授 (70135028)
ADACHI Isao Department of Hospital Pharmacy, Toyama Medical & Pharmaceutical University, Res, 附属病院, 助手 (30151070)
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| Project Period (FY) |
1985 – 1987
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| Project Status |
Completed (Fiscal Year 1987)
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| Budget Amount *help |
¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 1987: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1986: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1985: ¥1,900,000 (Direct Cost: ¥1,900,000)
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| Keywords | Calorimetry / Fibrinolytic activity / Microcalorimeter / Flow-throgh-Cell / Urokinase / Fibrinogen / Plasminogen / プラスミン / フィブリン / 活性定量 / 熱 / 活性 / 微少熱量計 |
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| Research Abstract |
A novel method using calormetry on the evaluation of activity of fibrinolytic enzyme was developed. LKB microcaolrimeter equipped by flow-throug-cell was used. The protocol of the method was as follows: An enzyme and an excess amount of the substrate were mixed. The solution, in which the fibrinolytic reaction was in the steady state, was led to a thermodetector. The heat produced in unit time was measured. First, the reaction heat in two component system was measuted. Plasmin was used as a fibrinolytic enzyme. The reaction heat for fibrinogen, fibrin and casein as substrates of plasmin were measured. As fibrinogen showed the highest reaction heat, fibrinogen was adoptted as the substrate. Next, 3 component system i.e. urokinase, plasminogen and fibrinogen, was studied. Because of the practical 0 heat of the reaction from plasmininogen to plasmin, the heat observed in this system can be regraded as that of the reaction from fibrinogen to FDP by Plasmin. A linear relationship between UK concentration and observed heat was obtaind within the UK concentration of 0.5-20 unit/ml. Thus the method on evaluation of fibrinolitic activity using calorimetry was established. The methods using vescoelasticity and light transmittance were also studied.
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