| Project/Area Number |
60870082
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| Research Category |
Grant-in-Aid for Developmental Scientific Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Biological pharmacy
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| Research Institution | Hokkaido University |
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Principal Investigator |
OHTSUKA Eiko Professor, Hokkaido University, Fac. of Pharmaceutical Science, 薬学部, 教授 (80028836)
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| Co-Investigator(Kenkyū-buntansha) |
IWAI Shigenori Research associate, Hokkaido University, Fac. of Pharmaceutical Science, 薬学部, 教務職員 (10168544)
INOUE Hideo Research associate, Hokkaido University, Fac. of Pharmaceutical Science, 薬学部, 助手 (80088856)
MIURA Kazunobu Research associate, Hokkaido University, Fac. of Pharmaceutical Science, 薬学部, 助手 (70001980)
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| Project Period (FY) |
1985 – 1986
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| Project Status |
Completed (Fiscal Year 1986)
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| Budget Amount *help |
¥6,000,000 (Direct Cost: ¥6,000,000)
Fiscal Year 1986: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1985: ¥4,000,000 (Direct Cost: ¥4,000,000)
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| Keywords | c-Ha-ras gene / Synthetic gene / Monoclonal antibodies / GDP binding activity / GTPase活性 / P21 / GDP結合蛋白質 |
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| Research Abstract |
The present invesigation intends a production of antibodies against products of c-Ha-ras genes. These antibodies can be applied to diagonosis of cancers. In order to prepare c-Ha-ras proteins, genes coding for normal and activated c-Ha-ras proteins were synthesized gene fragments coding for either 12-gly, 12-val or 61-lau. The synthetic genes were joined to downstream of the trp promoter in an expression vector and expressed in E.coli. The expressed proteins were isolated by ion-exchange chromatography and gel filtration in quantity. These proteins were used to produce monoclonal antibodies by injecting to mice and hybridization of their spleen cells with myeloma cells. Antibody producing cells were screened and cloned. Anti c-Ha-ras (val-12) obtained from a hybridoma recognized c-Ha-ras (val-12) as well as c-Ha-ras (gly-12). This antibody are to be used to develop a method to detect cancers overproducing c-Ha-ras proteins.
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