| Project/Area Number |
60880010
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| Research Category |
Grant-in-Aid for Developmental Scientific Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Laboratory animal science
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| Research Institution | Osaka University(Medical School) |
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Principal Investigator |
KITAMURA Yukihiko Osaka University Medical School, 医学部, 教授 (70028520)
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| Co-Investigator(Kenkyū-buntansha) |
FUJITA Jun Osaka Univesity Medical %shcool assistant provessor, 医学部, 助教授 (50173430)
NAKANO Taru Osaka University Medecal School assistant, 医学部, 助手 (00172370)
KANAKURA Yuzuru Osaka University Medical Shcool assistant, 医学部, 助手 (20177489)
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| Project Period (FY) |
1985 – 1987
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| Project Status |
Completed (Fiscal Year 1987)
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| Budget Amount *help |
¥13,500,000 (Direct Cost: ¥13,500,000)
Fiscal Year 1987: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1986: ¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1985: ¥9,000,000 (Direct Cost: ¥9,000,000)
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| Keywords | Mast cell-deficient nice / Mutant mouse / Animal model of human diseade / Mast cell / Cell culture / インターロイキン3 / 肥満細胞欠損動物 / 疾患モデル / アレルギー疾患 / インターリウキン / IgE / アレルギー / 血栓栓塞症 / ダニ |
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| Research Abstract |
Genetically mast cell-deficient W/W^v and Sl/Sl^d mice are useful for the analysis of mast cell biology. Especially, W/W^v mine are useful as recipients of bone marrow cells and skin pieces. Inasmuch as suspentsion and clonal cultures of mast cells have been developed, wwe combined these in vivio and in vitro systems.
1) Suspension-cultured mast cells had morphological and biochemical characteristics similar to those of mucosla mast cells (MMX). However, i.p. injection of such cultured mast cells bage rise to development of cells charactersitics similar to those of nonnective tissue mast cells (CTMC).
2) Hwen peritoneal cells of normal +/+ mice were cultured in methylcellulose, pure mast cell colonies appeared. Cells from individual mast cell colonies were divided and injected into the skin and stomach W/W^v mice; CTMC developed in the skin and MMC in the stomach mucosa. This indicates the presence of a common precursor for CTMC and MMC.
3) Morphology of such bipotent mast cell precustors was studied by using micromanipultaion About 4% of morphologically identifiable peritineal mast cells may function as the bipotent precursors,
4) Although W/W^v mice showed a defect in resistance against ixodid ticks, injection of suspension-cultured mast cells normalized the defect. The examples mentioned above indicate that combinations if in vivo and in vitro systems increara the usedfulness of W/W^v mice.
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