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Development of the method for systematic analysis of the sugar chains of glycoproteins with use of immobilized lectin columns.

Research Project

Project/Area Number 60880019
Research Category

Grant-in-Aid for Developmental Scientific Research

Allocation TypeSingle-year Grants
Research Field 物質生物化学
Research InstitutionInstitute of Medical Science, University of Tokyo

Principal Investigator

KOBATA Akira  Institute of Medical Science, University of Tokyo, 医科学研究所, 教授 (30030852)

Co-Investigator(Kenkyū-buntansha) KOCHIBE Naohisa  Faculty of Education, Gunma University, 教育学部, 助教授 (60008266)
ENDO Tamao  Institute of Medical Science, University of Tokyo, 医科学研究所, 助手 (30168827)
YAMASHITA Katsuko  Kobe University School of Medicine, 医学部, 助教授 (70030905)
Project Period (FY) 1985 – 1986
Project Status Completed (Fiscal Year 1986)
Budget Amount *help
¥9,100,000 (Direct Cost: ¥9,100,000)
Fiscal Year 1986: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1985: ¥7,100,000 (Direct Cost: ¥7,100,000)
KeywordsLectin / Oligosaccharides / Affinity column chromatography / Glycoprotein / 系統分析 / 植物性凝集素 / 親和性クロマト
Research Abstract

1. The methods to obtain large amount of AAL (Aleuria aurantia lectin) and DSA (Datura stramonium agglutinin) were established.
2. By investigating the behaviors of various asparagine-linked oligosaccharides on the immobilized AAL and DSA columns, the binding specificities of these two lectin columns were elucidated.
3. AAL-Sepharose column was useful for the group separation of asparagine-linked sugar chains by the presence or absence of a fucose residue linked at the C-6 position of proximal N-acetylglucosamine residue.
4. DSA-Sepharose is useful for separating tri- and tetraantennary oligosaccharides because those with the Gal <beta> 1->4GlcNAc <beta> 1->4(Gal <beta> 1->4GlcNAc <beta> 1->2)Man group are retarded on the column, while those with the Gal <beta> 1->4GlcNAc <beta> 1->6(Gal <beta> 1->4 GlcNAc <beta> 1->2)Man group bind and eluted with buffer containing chitotetraose.
5. By sequential passage through AAL-, RCAI-, E-PHA, and Con A columns, 16 different biantennary complex-type sugar chains released from IgG can completely be separated.

Report

(2 results)
  • 1986 Final Research Report Summary
  • 1985 Annual Research Report
  • Research Products

    (15 results)

All Other

All Publications (15 results)

  • [Publications] Yamashita,K.;Kochibe,N.;Kobata,A.: J.Biol.Chem.260. 4689-4693 (1985)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Taniguchi,T.;Kochibe,N.;Kobata,A.: J.Biol.Chem.261. 1730-1736 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Endo,T.;Kobata,A.: Carbohyd.Res.150. 241-263 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Takasaki,S.,Kobata,A.: Biochemistry. 25. 5709-5715 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Yamashita,K.;Kobata,A.: Arch.Biochem.Biophys.250. 263-266 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Yamashita,K.;Kobata,A.: J.Biol.Chem.250. (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Yamashita, K., Kochibe, N., Kobata, A.: "Fractionation of L-fucose-containing oligosaccharides on immobilized Aleuria aurantia lectin." J. Biol. Chem.260. 4689-4693 (1985)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Taniguchi, T., Kochibe, N., Kobata, A.: "The structures of the asparagine-linked sugar chains of bovine interphotoreceptor retinol-binding protein: Occurrence of fucosylated hybrid-type oligosaccharides." J. Biol. Chem.261. 1730-1736 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Endo, T., Kobata, A.: " <beta> -Galactosyltransferase from human milk contains a variety of complex-type asparagine-linked sguar chains enriched with X and dimeric X in their outer chains." Carbohyd. Res.150. 241-263 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Takasaki, S., Kobata, A.: "Asparagine-linked sugar chains of fetuin: Occurrence of tetrasialyl triantennary sugar chains containing the Gal <beta> 1->3GlcNAc sequence." Biochemistry. 25. 5709-5715 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Yamashita, K., Kobata, A.: "Fractionation with a variety of lectin affinity chromatographies indicated that most ribonucleases in human viscera and body fluid are glycosylated in organ-specific manner." Arch. Biochem. Biophys.250. 263-266 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] J.Biol.Chem.260-8. (1985)

    • Related Report
      1985 Annual Research Report
  • [Publications] Glycoconjugate J.2-2. (1985)

    • Related Report
      1985 Annual Research Report
  • [Publications] Jpn.J.Exp.Med.55-2. (1985)

    • Related Report
      1985 Annual Research Report
  • [Publications] Biochemistry. 24-2. (1985)

    • Related Report
      1985 Annual Research Report

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Published: 1987-03-31   Modified: 2016-04-21  

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